ULTRASTRUCTURAL STUDIES OF BACTERIA IN ANAEROBIC BIOMASS DIGESTERS

被引:7
作者
ALDRICH, HC
机构
[1] Department of Microbiology and Cell Science, University of Florida, Gainesville
关键词
METHANOGENIC BACTERIA; METHANOSARCINA-BARKERI; METHANOSARCINA-MAZEI; METHANOBACTERIUM-THERMOAUTOTROPHICUM; METHANOTHRIX-CONCILII; IMMUNOGOLD; ULTRASTRUCTURE;
D O I
10.1016/0961-9534(93)90074-E
中图分类号
S2 [农业工程];
学科分类号
0828 ;
摘要
Electron microscopy has been used to study the morphology of Methanosarcina mazei and Methanobacterium thermoautotrophicum. Internal membranes were shown to be fixation artifacts. Immunogold techniques were used to identify bacterial species in anaerobic biomass digesters and to determine the cellular locations of enzymes important in methanogenesis. Invasion of and destruction of the cell wall of plant tissue in a digester by cellulolytic bacteria are also illustrated.
引用
收藏
页码:241 / 246
页数:6
相关论文
共 15 条
[1]  
Robinson, Aldrich, Hurst, Bleiweis, Role of the cell surface of Methanosarcina mazei in cell aggregation, Appl. Env. Microbiol., 49, pp. 321-327, (1985)
[2]  
Aldrich, Robinson, Williams, Ultrastructure of Methanosarcina mazei, Systematic and Applied Microbiology, 7, pp. 314-319, (1986)
[3]  
Bleiweis, Aldrich, Mah, Properties that affect aggregation and dispersion of methanogens, Methane from Biomass. A Systems Approach, pp. 397-411, (1988)
[4]  
Aldrich, Williams, Robinson, Ultrastructural analyses of methanogens, Methane from Biomass: A Systems Approach, pp. 385-396, (1988)
[5]  
Aldrich, Beimborn, Schonheit, Creation of artifactual internal membranes during fixation of Methanobacterium thermoautotrophicum, Canadian Journal of Microbiology, 33, pp. 844-849, (1987)
[6]  
Sewell, Aldrich, Williams, Mannarelli, Wilkie, Hespell, Smith, Ingram, Isolation and characterization of xylan-degrading strains of Butyrivibrio fibrisolvens from a Napier grass-fed anaerobic digester, Appl. Env. Microbial., 54, pp. 1085-1090, (1988)
[7]  
Geerligs, Aldrich, Harder, Diekert, Isolation and characterization of a carbon monoxide utilizing strain of the acetogen Peptostreptococcus productus, Arch. Microbial., 148, pp. 305-313, (1987)
[8]  
Kell, Doddema, Morris, Vogels, Energy coupling in methanogens, Proc. Int. Symp. 3rd, pp. 159-170, (1981)
[9]  
Hasty, Hay, Freeze fracture studies of the developing cell surface. II. Particle free membrane blisters on glutaraldehyde-fixed corneal fibroblasts are artefacts, J. Cell Biol., 78, pp. 756-768, (1978)
[10]  
Robinson, Erdos, Immuno-electron microscopic identification of Methanosarcina spp. in anaerobic digester fluid, Can. J. Microbial., 31, pp. 839-844, (1985)