MERCURY AND TETRACYCLINE RESISTANCE GENES AND FLANKING REPEATS ASSOCIATED WITH METHICILLIN RESISTANCE ON THE CHROMOSOME OF STAPHYLOCOCCUS-AUREUS

被引：39

作者：

SKINNER, S ^{[1
]}

INGLIS, B ^{[1
]}

MATTHEWS, PR ^{[1
]}

STEWART, PR ^{[1
]}

机构：

[1] AUSTRALIAN NATL UNIV, FAC SCI, DEPT BIOCHEM, CANBERRA, ACT 2601, AUSTRALIA

来源：

MOLECULAR MICROBIOLOGY | 1988年 / 2卷 / 02期

关键词：

D O I：

10.1111/j.1365-2958.1988.tb00030.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Sections of a cloned 27 kb segment of chromosomal DNA, associated with resistance to four antimicrobial agents in a clinical isolate of methicillin-resistant Staphylococcus aureus (MRSA), were tested for their ability to determine resistance when transformed into a sensitive laboratory strain of S. aureus. This was achieved by inserting the sections in to a newly constructed shuttle vector, amplifying the recombinant DNA in E. coli, and transforming protoplasts of the sensitive S. aureus strain. Two sections of the cloned DNA were found to determined resistance separately to mercuric ion and to tetracycline, in both S. aureus and Escherichia coli.

引用

页码：289 / 292

页数：4

共 15 条

[1] IS431, A STAPHYLOCOCCAL INSERTION SEQUENCE-LIKE ELEMENT RELATED TO IS26 FROM PROTEUS-VULGARIS
BARBERISMAINO, L
BERGERBACHI, B
WEBER, H
BECK, WD
KAYSER, FH
[J]. GENE, 1987, 59 (01) : 107 - 113
[2] ADDITIONAL DNA IN METHICILLIN-RESISTANT STAPHYLOCOCCUS-AUREUS AND MOLECULAR-CLONING OF MEC-SPECIFIC DNA
BECK, WD
BERGERBACHI, B
KAYSER, FH
[J]. JOURNAL OF BACTERIOLOGY, 1986, 165 (02) : 373 - 378
[3] BETLEY MJ, 1986, ANNU REV MICROBIOL, V40, P577, DOI 10.1146/annurev.mi.40.100186.003045
[4] DORNBUSCH K, 1973, J GEN MICROBIOL, V76, P1
[5] PLASMID-CHROMOSOMAL TRANSITION OF GENES IMPORTANT IN STAPHYLOCOCCAL ENTEROTOXIN-B EXPRESSION
DYER, DW
IANDOLO, JJ
[J]. INFECTION AND IMMUNITY, 1981, 33 (02) : 450 - 458
[6] GILLESPIE MT, 1986, J GEN MICROBIOL, V132, P1723
[7] HOMOLOGOUS DIRECT REPEAT SEQUENCES ASSOCIATED WITH MERCURY, METHICILLIN, TETRACYCLINE AND TRIMETHOPRIM RESISTANCE DETERMINANTS IN STAPHYLOCOCCUS-AUREUS
GILLESPIE, MT
LYON, BR
LOO, LSL
MATTHEWS, PR
STEWART, PR
SKURRAY, RA
[J]. FEMS MICROBIOLOGY LETTERS, 1987, 43 (02) : 165 - 171
[8] INGLIS B, 1988, IN PRESS J GEN MICRO
[9] LADAGGA RA, 1987, P NATL ACAD SCI USA, V84, P5106
[10] TN4001 - A GENTAMICIN AND KANAMYCIN RESISTANCE TRANSPOSON IN STAPHYLOCOCCUS-AUREUS
LYON, BR
MAY, JW
SKURRAY, RA
[J]. MOLECULAR & GENERAL GENETICS, 1984, 193 (03): : 554 - 556

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