EVALUATION OF A RETENTION MODEL FOR HIGH-PERFORMANCE ION-EXCHANGE CHROMATOGRAPHY USING 2 DIFFERENT DISPLACING SALTS

被引：96

作者：

ROUNDS, MA ^{[1
]}

REGNIER, FE ^{[1
]}

机构：

[1] PURDUE UNIV, DEPT BIOCHEM, W LAFAYETTE, IN 47907 USA

来源：

JOURNAL OF CHROMATOGRAPHY | 1984年 / 283卷 / JAN期

关键词：

D O I：

10.1016/S0021-9673(00)96240-X

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A model proposed for the retention of proteins on high-performance ion-exchange chromatographic supports was further investigated via the use of NaCl vs. MgCl2 as the chromatographic displacing salt. Evaluations of the Z term [number of charged groups involved in the adsorption/desorption process] generated by this model were carried out using selected proteins on a strong anion-exchange support in an effort to define its meaning more clearly. A positive correlation between Z and protein retention was found. Higher values for ZMgCl2 were attributed to enhanced ionization of protein functional groups of Mg2+. Findings suggest that Z could be more precisely defined as the number of electrostatic interactions between the protein and the ion-exchange support.

引用

页码：37 / 45

页数：9

共 20 条

[1] A PMR STUDY OF THE EFFECTS OF PH AND ANION AND METAL-ION BINDING OF THE HISTIDYL RESIDUES OF OVOTRANSFERRIN
ALSAADI, BM
WILLIAMS, RJP
WOODWORTH, RC
[J]. JOURNAL OF INORGANIC BIOCHEMISTRY, 1981, 15 (01) : 1 - 10
[2] BINDING OF SODIUM IONS TO BETA-LACTOGLOBULIN
BAKER, HP
SAROFF, HA
[J]. BIOCHEMISTRY, 1965, 4 (08) : 1670 - &
[3] CANNAN R. KEITH, 1941, ANN NEW YORK ACAD SCI, V41, P243, DOI 10.1111/j.1749-6632.1941.tb35241.x
[4] SODIUM ION BINDING TO PEPSIN AND OTHER PROTEINS
CLEMENT, GE
SIEGEL, A
POTTER, R
[J]. CANADIAN JOURNAL OF BIOCHEMISTRY, 1971, 49 (05): : 477 - &
[5] Huheey J.E., 1972, Inorganic chemistry, principles of structure and reactivity, P214
[6] STUDIES ON SOYBEAN TRYPSIN-INHIBITORS .1. FRAGMENTATION OF SOYBEAN TRYPSIN-INHIBITOR (KUNITZ) BY LIMITED PROTEOLYSIS AND BY CHEMICAL CLEAVAGE
KOIDE, T
IKENAKA, T
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1973, 32 (03): : 401 - 407
[7] RETENTION MODEL FOR HIGH-PERFORMANCE ION-EXCHANGE CHROMATOGRAPHY
KOPACIEWICZ, W
ROUNDS, MA
FAUSNAUGH, J
REGNIER, FE
[J]. JOURNAL OF CHROMATOGRAPHY, 1983, 266 (AUG): : 3 - 21
[8] KOPACIEWICZ W, 1983, ANAL BIOCH
[9] CRYSTALLINE SOYBEAN TRYPSIN INHIBITOR .2. GENERAL PROPERTIES
KUNITZ, M
[J]. JOURNAL OF GENERAL PHYSIOLOGY, 1947, 30 (04) : 291 - 310
[10] LASKOWSKI M, COMMUNICATION

← 1 2 →