VIROSOMES RECONSTITUTED FROM HUMAN-IMMUNODEFICIENCY-VIRUS PROTEINS AND LIPIDS

被引：14

作者：

CORNET, B ^{[1
]}

VANDENBRANDEN, M ^{[1
]}

COGNIAUX, J ^{[1
]}

GIURGEA, L ^{[1
]}

DEKEGEL, D ^{[1
]}

RUYSSCHAERT, JM ^{[1
]}

机构：

[1] INST PASTEUR,B-1180 BRUSSELS,BELGIUM

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 167卷 / 01期

关键词：

D O I：

10.1016/0006-291X(90)91754-G

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Purified Human Immunodeficiency Virus (HIV) was solubilized in octylglucopyranoside. After centrifugation, the supernatant was added to lipid-detergent mixed micelles. Formation of virosomes occurred during overnight dialysis. Centrifugation on a continuous glycerol gradient showed that envelope glycoproteins (gp120 and gp41) and matrix protein p17 but not core protein p25 were associated to virosomes. Proteolytic treatment of virosomes indicates that gp120 is oriented toward the outside as in the virus particles, whereas p17 protein is anchored on both sides of the liposomal membrane. Virosomes are spherical vesicles with approximately the size of the virus as shown by electron microscopy. © 1990.

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收藏

页码：222 / 231

页数：10

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