CONCANAVALIN-A STIMULATES THE ROLIPRAM-SENSITIVE ISOFORMS OF CYCLIC-NUCLEOTIDE PHOSPHODIESTERASE IN RAT THYMIC LYMPHOCYTES

被引：33

作者：

VALETTE, L

PRIGENT, AF

NEMOZ, G

ANKER, G

MACOVSCHI, O

LAGARDE, M

机构：

[1] Unité INSERM 205, Laboratoire de Chimie Biologique, Institut National des Sciences Appliquées de Lyon, 69621 Villeurbanne Cédex, Bat 406

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 169卷 / 03期

关键词：

D O I：

10.1016/0006-291X(90)91973-V

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Pretreatment of rat thymic lymphocytes with Concanavalin A induced a very early (30 min) and substantial increase (+90%) of the soluble cAMP phosphodiesterase activity. The crude cytosolic phosphodiesterase activity of rat thymocytes could reproducively be resolved by Mono-Q ion exchange high performance liquid chromatography into four separate phosphodiesterase peaks: a cGMP-stimulated, two cAMP-specific Rolipram-sensitive and a cGMP-inhibited cardiotrope-sensitive peaks. Concanavalin A stimulated very specifically the activity of the two predominant cAMP-specific Rolipram sensitive peaks whereas it only slightly modified the cGMP-stimulated and the cGMP-inhibited forms. The present results strongly suggest that the Rolipram-sensitive cAMP PDE activity may play a key role in the control and regulation of mitogen-induced thymocyte proliferation. © 1990.

引用

页码：864 / 872

页数：9

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