A RELIABLE METHOD FOR THE AMINO-ACID-SEQUENCE DETERMINATION IN TETRAPEPTIDES - A STUDY ON THE POSITIVE AND NEGATIVE-ION TANDEM MASS-SPECTRA OF TETRAPEPTIDES

被引：15

作者：

KULIK, W

HEERMA, W

机构：

[1] University of Utrecht, Faculty of Chemistry, Bijvoet Centre for Biomolecular Research, Utrecht, 3505 TB

来源：

BIOLOGICAL MASS SPECTROMETRY | 1991年 / 20卷 / 09期

关键词：

D O I：

10.1002/bms.1200200908

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

An unambiguous method for sequencing tetrapeptides based upon the combined use of fast atom bombardment mass, MI and collisional activation (CA) spectra of protonated and sodium-cationized peptide molecules is proposed. The positions in the peptide of non-C-terminal Ile/Leu are determined by 'W(n) + Na ions in the CA spectra of [M + Na]+. The second part of the paper is an evaluation of the fragmentations in the negative ion MI and CA spectra of deprotonated tetrapeptides.

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页码：553 / 558

页数：6

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