CLONING AND MAPPING OF THE CYS4 GENE OF SACCHAROMYCES-CEREVISIAE

被引：11

作者：

ONO, B ^{[1
]}

HEIKE, C ^{[1
]}

YANO, Y ^{[1
]}

INOUE, T ^{[1
]}

NAITO, K ^{[1
]}

NAKAGAMI, S ^{[1
]}

YAMANE, A ^{[1
]}

机构：

[1] WAKUNAGA PHARMACEUT CO LTD,INST BIOTECHNOL RES,KODA,HIROSHIMA 72964,JAPAN

来源：

CURRENT GENETICS | 1992年 / 21卷 / 4-5期

关键词：

SACCHAROMYCES-CEREVISIAE; CYSTEINE BIOSYNTHETIC; CYS4; MAPPING;

D O I：

10.1007/BF00351684

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A DNA fragment containing the CYS4 gene of Saccharomyces cerevisiae was isolated from a genomic library. The cloned fragment hybridized to the transverse-alternating-field-electrophoresis band corresponding to chromosomes VII and XV. According to the 2-mu-m DNA chromosome-loss procedure, the cys2 and cys4 mutations, which are linked together and co-operatively confer cysteine dependence, were assigned to chromosome VII. By further mapping involving tetrad analysis, the cys2-cys4 pair was localized between SUP77 (SUP166) and ade3 on the right arm of chromosome VII.

引用

页码：285 / 289

页数：5

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