POTENT ACTIVATION OF PHOSPHOLIPASE-D BY PHENYLARSINE OXIDE IN RAT BASOPHILIC LEUKEMIA (RBL-2H3) CELLS

被引:17
作者
KUMADA, T
NAKASHIMA, S
MIYATA, H
NOZAWA, Y
机构
[1] GIFU UNIV,SCH MED,DEPT BIOCHEM,GIFU 500,JAPAN
[2] GIFU UNIV,SCH MED,DEPT OTOLARYNGOL,GIFU 500,JAPAN
关键词
D O I
10.1006/bbrc.1994.1299
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A putative protein tyrosine phosphatase inhibitor, phenylarsine oxide (PAO),potentiated phospholipase D (PLD) activity concentration-dependently in [H-3] oleic acid-labeled rat basophilic leukemia (RBL-2H3) cells without significant increase in phosphatidylinositol-specific plospholipase C (PI-PLC) activity. Although PAO induced tyrosine phosphorylation of several proteins, both PAO-induced PLD activation and tyrosine phosphorylation were not affected by a protein tyrosine kinase inhibitor, genistein. Another tyrosine kinase inhibitor, herbimycin A, prevented the PAO-induced PLD stimulation but had no effect on protein tyrosine phosphorylation. However, depletion of protein kinase C (PKC) greatly reduced PAO-stimulated PLD activity. These results indicate that PKC but not tyrosine kinase may be involved in PAO-mediated PLD activation. (C) 1994 Academic Press, Inc.
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页码:792 / 798
页数:7
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