CHARACTERIZATION OF 3 HUMAN MONOCLONAL-ANTIBODIES SPECIFIC FOR POLYMORPHIC CLASS-I AND CLASS-II HLA ANTIGENS

被引：13

作者：

DESSI, V ^{[1
]}

SANCHEZ, B ^{[1
]}

GARZON, M ^{[1
]}

MAGARINO, R ^{[1
]}

MALDONADO, MD ^{[1
]}

NUNEZROLDAN, A ^{[1
]}

机构：

[1] HOSP UNIV VIRGEN ROCIO,SERV ANDALUZ SALUD,E-41013 SEVILLE,SPAIN

来源：

HUMAN IMMUNOLOGY | 1990年 / 27卷 / 04期

关键词：

D O I：

10.1016/0198-8859(90)90083-2

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Three human monoclonal antibodies were derived from a single polytransfused patient awaiting renal transplantation. In microcitotoxicity assays, the patient's serum displayed strong positive reactions against > 90% of a panel of cells representing the known HLA specificities. The donor's peripheral blood lymphocytes were infected with Epstein-Barr virus, cloned, and supernatants of the virus transformed cultures were screened for the presence of IgG antilymphocyte reactivity utilizing an enzyme-linked immunosorbent assay method. Positive cultures were recloned and fused with the human-mouse heteromyeloma SHM. Supernatants from three clones were selected for alloreactivity and characterized by indirect immunofluorescent staining and fluoractivated cell sorter analysis on homozygous typing cells, including those from the Tenth International Histocompatibility Workshop core panel and on cell lines derived from selected families. Data obtained demonstrate that two human monoclonal antibodies have DQw1 specificity, one of them being reactive against several DQw7-positive cell lines, while one monoclonal antibody is specific for the A2 + A28 class I MHC antigens. Anti-DQw1 antibodies were of different light-chain subtypes. © 1990.

引用

页码：323 / 332

页数：10

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