RECYCLING OF EPIDERMAL GROWTH FACTOR-RECEPTOR COMPLEXES IN A431 CELLS - IDENTIFICATION OF DUAL PATHWAYS

被引:98
作者
SORKIN, A [1 ]
KROLENKO, S [1 ]
KUDRJAVTCEVA, N [1 ]
LAZEBNIK, J [1 ]
TESLENKO, L [1 ]
SODERQUIST, AM [1 ]
NIKOLSKY, N [1 ]
机构
[1] VANDERBILT UNIV,MED CTR,SCH MED,DEPT BIOCHEM,NASHVILLE,TN 37232
关键词
D O I
10.1083/jcb.112.1.55
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The intracellular sorting of EGF-receptor complexes (EGF-RC) has been studied in human epidermoid carcinoma A431 cells. Recycling of EGF was found to occur rapidly after internalization at 37-degrees-C. The initial rate of EGF recycling was reduced at 18-degrees-C. A significant pool of internalized EGF was incapable of recycling at 18-degrees-C but began to recycle when cells were warmed to 37-degrees-C. The relative rate of EGF outflow at 37-degrees from cells exposed to an 18-degrees-C temperature block was slower (t1/2 almost-equal-to 20 min) than the rate from cell s not exposed to a temperature block (t1/2 almost-equal-to 5-7 min). These data suggest that there might be both short- and long-time cycles of EGF recycling in A431 cells. Examination of the intracellular EGF-RC dissociation and dynamics of short- and long-time recycling indicated that EGF recycled as EGF-RC>. Moreover, EGF receptors that were covalently labeled with a photoactivatable derivative of I-125-EGF recycled via the long-time pathway at a rate similar to that of I-125-EGF. Since EGF-RC degradation was also blocked at 18-degrees-C, we propose that sorting to the lysosomal and long-time recycling pathway may occur after a highly temperature-sensitive step, presumably in the late endosomes.
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页码:55 / 63
页数:9
相关论文
共 43 条
[1]   UPTAKE AND INTRACELLULAR PROCESSING OF EPIDERMAL-GROWTH-FACTOR RECEPTOR COMPLEXES [J].
BEARDMORE, J ;
HOPKINS, CR .
BIOCHEMICAL SOCIETY TRANSACTIONS, 1984, 12 (02) :165-168
[2]   DOWN-REGULATION OF THE EPIDERMAL GROWTH-FACTOR RECEPTOR IN KB CELLS IS DUE TO RECEPTOR INTERNALIZATION AND SUBSEQUENT DEGRADATION IN LYSOSOMES [J].
BEGUINOT, L ;
LYALL, RM ;
WILLINGHAM, MC ;
PASTAN, I .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (08) :2384-2388
[3]   PHORBOL ESTERS INDUCE TRANSIENT INTERNALIZATION WITHOUT DEGRADATION OF UNOCCUPIED EPIDERMAL GROWTH-FACTOR RECEPTORS [J].
BEGUINOT, L ;
HANOVER, JA ;
ITO, S ;
RICHERT, ND ;
WILLINGHAM, MC ;
PASTAN, I .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (09) :2774-2778
[4]  
BEGUINOT L, 1986, J BIOL CHEM, V261, P1801
[5]  
BURGESS AW, 1983, EMBO J, V2, P2062
[6]  
CARPENTER G, 1987, ANNU REV BIOCHEM, V56, P881, DOI 10.1146/annurev.bi.56.070187.004313
[7]   DIRECT VISUALIZATION OF THE PHOSPHORYLATED EPIDERMAL GROWTH-FACTOR RECEPTOR DURING ITS INTERNALIZATION IN A-431 CELLS [J].
CARPENTIER, JL ;
WHITE, MF ;
ORCI, L ;
KAHN, RC .
JOURNAL OF CELL BIOLOGY, 1987, 105 (06) :2751-2762
[8]  
CHINKERS M, 1986, J BIOL CHEM, V261, P8295
[9]   DYNAMIC BEHAVIOR OF THE TRANSFERRIN RECEPTOR FOLLOWED IN LIVING EPIDERMOID CARCINOMA (A431) CELLS WITH NANOVID MICROSCOPY [J].
DEBRABANDER, M ;
NUYDENS, R ;
GEERTS, H ;
HOPKINS, CR .
CELL MOTILITY AND THE CYTOSKELETON, 1988, 9 (01) :30-47
[10]   RECEPTOR-MEDIATED ENDOCYTOSIS OF EPIDERMAL GROWTH-FACTOR BY RAT HEPATOCYTES - RECEPTOR PATHWAY [J].
DUNN, WA ;
CONNOLLY, TP ;
HUBBARD, AL .
JOURNAL OF CELL BIOLOGY, 1986, 102 (01) :24-36