CAPILLARY ELECTROPHORESIS LASER FLUOROMETRY INSTRUMENTATION FOR THE FACILE OPTIMIZATION OF DNA SEPARATIONS USING IN-SITU SIZE-SELECTIVE GRADIENTS AND ADJUSTABLE DETECTION ZONE

被引：15

作者：

CLARK, BK ^{[1
]}

VO-DINH, T ^{[1
]}

SEPANIAK, MJ ^{[1
]}

机构：

[1] UNIV TENNESSEE, DEPT CHEM, KNOXVILLE, TN 37996 USA

来源：

ANALYTICAL CHEMISTRY | 1995年 / 67卷 / 03期

关键词：

D O I：

10.1021/ac00099a030

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The construction and evaluation of a capillary electrophoresis instrument for the facile optimization of DNA separations is described herein. This instrumentation allows for convenient translation of the detection zone with little loss in detectability. Changing the effective capillary length is shown to be important when complete resolution of specified DNA fragments in a sample, in the minimum time, is desired, Efficiency is found to decrease with column length in accordance with limits imposed by axial diffusion. However, complete resolution of the fragments in a Phi X-174-HeaIII digest is accomplished in 155 s at moderate held strengths and an effective capillary length of 15 cm. It is also demonstrated that in situ gradients can be established using this instrumentation. The general chromatographic elution problem is addressed with gradients, resulting in further improvements in separation performance relative to isocratic separations.

引用

页码：680 / 683

页数：4

共 21 条

[1] RAPID SEPARATION OF DNA RESTRICTION DIGESTS USING SIZE-SELECTIVE CAPILLARY ELECTROPHORESIS WITH APPLICATION TO DNA-FINGERPRINTING [J].