A NEW ASSAY FOR FATTY ACID DESATURATION

被引：26

作者：

TALAMO, BR

BLOCH, K

机构：

[1] The James Bryant Conant Laboratory, Harvard University, Cambridge

来源：

ANALYTICAL BIOCHEMISTRY | 1969年 / 29卷 / 02期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1016/0003-2697(69)90313-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The enzymic formation of unsaturated fatty acids from their saturated homologs is ordinarily assayed by gas-liquid chromatography or by chromatography of the complexes formed between the olefinic acids and mercuric acetate or silver nitrate. In the present paper a rapid, sensitive assay is described. It is based on the release of tritium as tritiated water from a substrate specifically labeled at the positions to be desaturated. The method circumvents the time consuming operations (saponification, extraction, esterification, and chromatography) of the older procedures. A similar principle has been utilized for assaying aromatic hydroxylation (1). Both CoA and acyl carrier protein (ACP) fatty acid thioesters are precipitable by acid and can be collected on a Millipore filter which is conveniently attached to a syringe. The tritiated water filtrate is collected and an aliquot counted. The sensitivity of the method extends to the picomole range and is limited only by the specific activity of the substrate. © 1969.

引用

页码：300 / &

共 10 条

[1] CHEMISTRY OF DIIMIDE .2. STEREOCHEMISTRY OF HYDROGEN TRANSFER TO CARBON-CARBON MULTIPLE BONDS [J].