SELECTIVE ISOTOPIC LABELING OF A NITROXIDE SPIN LABEL TO ENHANCE SENSITIVITY FOR T2 OXYMETRY

被引:70
作者
HALPERN, HJ
PERIC, M
NGUYEN, TD
SPENCER, DP
TEICHER, BA
LIN, YJ
BOWMAN, MK
机构
[1] HARVARD UNIV,SCH MED,DANA FARBER CANC INST,BOSTON,MA 02115
[2] ARGONNE NATL LAB,DIV CHEM,ARGONNE,IL 60439
来源
JOURNAL OF MAGNETIC RESONANCE | 1990年 / 90卷 / 01期
关键词
D O I
10.1016/0022-2364(90)90364-F
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The synthesis and application of a novel compound, a variant of a standard spin label which has been used for T2-based oxymetry, is described. The compound is 4-hydro-3carbamoyl-2,2,5,5-tetraperdeuteromethylpyrrolin-1-yloxy-d12 (mHCTPO). It was developed to optimize both the sensitivity of the T2 method to oxygen tension and the spectrometer signal for a given concentration of spin label. The compound is used with a very low frequency (250 MHz) electron paramagnetic resonance spectrometer for in vivo application and for imaging, but has application at X band. The compound provides a convenient spectral feature distinguishing broadening associated with self-interaction from that due to environmental oxygen. © 1990.
引用
收藏
页码:40 / 51
页数:12
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