THE MAJOR CAROTENOID PIGMENT OF A PSYCHROTROPHIC MICROCOCCUS-ROSEUS STRAIN - PURIFICATION, STRUCTURE, AND INTERACTION WITH SYNTHETIC MEMBRANES

The major carotenoid pigment of a psychrotrophic Micrococcus roseus strain was purified to homogeneity from methanol extracts of dried cells by reverse-phase liquid chromatography and was designated P-3. On the basis of the UV-visible, infrared, mass, and H-1 nuclear magnetic resonance spectra of P-3, it was identified as bisdehydro-beta-carotene-2-carboxylic acid. The pigment interacted with synthetic membranes of phosphatidylcholine and dimyristoyl phosphatidylcholine and stabilized the membranes. These results also indicate that P-3 is different from canthaxanthin, the major carotenoid pigment from a mesophilic M. roseus strain.