A MODIFIED KANAMYCIN-RESISTANCE CASSETTE TO FACILITATE 2-CODON INSERTION MUTAGENESIS

被引：10

作者：

CHEN, WJ ^{[1
]}

GROSS, L ^{[1
]}

JOHO, KE ^{[1
]}

MCALLISTER, WT ^{[1
]}

机构：

[1] SUNY HLTH SCI CTR,DEPT MICROBIOL & IMMUNOL,450 CLARKSON AVE,BROOKLYN,NY 11203

来源：

GENE | 1992年 / 111卷 / 01期

关键词：

RECOMBINANT DNA; TARGET PLASMID; PROTEIN ENGINEERING; LINKER INSERTION MUTAGENESIS;

D O I：

10.1016/0378-1119(92)90617-X

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A kanamycin-resistance (Km(R)) cassette was modified to allow its introduction as a blunt-ended DNA fragment into target plasmids. Subsequent excision of the Km(R) cassette with ApaI and religation of the plasmid leaves behind six base pairs (5'-GGGCCC) that result in the insertion of two amino acids in the target protein.

引用

页码：143 / 144

页数：2

共 6 条

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[5] CONSTRUCTION OF LINKER-SCANNING MUTATIONS USING A KANAMYCIN-RESISTANCE CASSETTE WITH MULTIPLE SYMMETRIC RESTRICTION SITES
SMITH, ML
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[6] MAPPING CATALYTICALLY IMPORTANT REGIONS OF AN ENZYME USING 2-CODON INSERTION MUTAGENESIS - A CASE-STUDY CORRELATING BETA-LACTAMASE MUTANTS WITH THE 3-DIMENSIONAL STRUCTURE
ZEBALA, J
BARANY, F
[J]. GENE, 1991, 100 : 51 - 57

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