SELECTION OF BACTERIAL VIRULENCE GENES THAT ARE SPECIFICALLY INDUCED IN HOST TISSUES

被引：529

作者：

MAHAN, MJ

SLAUCH, JM

MEKALANOS, JJ

机构：

[1] Dept. Microbiol. and Molec. Genet., Harvard Medical School, Boston

来源：

SCIENCE | 1993年 / 259卷 / 5095期

关键词：

D O I：

10.1126/science.8430319

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A genetic system was devised that positively selects for bacterial genes that are specifically induced when bacteria infect their host. With the pathogen Salmonella typhimurium, the genes identified by this selection show a marked induction in bacteria recovered from mouse spleen. Mutations in all ivi (in vivo-induced) genes that were tested conferred a defect in virulence. This genetic system was designed to be of general use in a wide variety of bacterial-host systems and has several applications in both vaccine and antimicrobial drug development.

引用

页码：686 / 688

页数：3

共 24 条

[1] ISOLATION AND PHENOTYPIC CHARACTERIZATION OF VIRULENCE-DEFICIENT MUTANTS OF VIBRIO-CHOLERAE [J].