THE GENE ENCODING RAT INSULIN-LIKE GROWTH FACTOR-BINDING PROTEIN-1 IS RAPIDLY AND HIGHLY INDUCED IN REGENERATING LIVER

被引:115
作者
MOHN, KL
MELBY, AE
TEWARI, DS
LAZ, TM
TAUB, R
机构
[1] UNIV PENN,SCH MED,DEPT HUMAN GENET,PHILADELPHIA,PA 19104
[2] UNIV PENN,SCH MED,HOWARD HUGHES MED INST,PHILADELPHIA,PA 19104
关键词
D O I
10.1128/MCB.11.3.1393
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The liver is an epithelioid organ that can regenerate following partial hepatectomy. Although it is composed mainly of hepatocytes, it has a complex, multicellular architecture, implying that intercellular communications must exist during regeneration. As in other mitogen-stimulated cells, immediate-early growth response genes induced in the absence of prior protein synthesis are likely to play an important regulatory role in the regenerative process. Through differential screening of regenerating liver cDNA libraries, we found that one of the most highly expressed immediate-early genes in liver regeneration encodes that rat homolog of the low-molecular-weight insulinlike growth factor (IGF)-binding protein (IGFBP-1). This protein has been implicated in enhancing the mitogenic effect of IGF on tissues. IGFBP-1 gene induction is transcriptionally mediated and specific to regenerating liver, as the gene is not expressed in mitogen-stimulated fibroblasts. IGFBP-1 expression has been shown to increase under low-insulin conditions such as diabetes, and the complex regulation of expression is indicated by our finding that insulin treatment of H35 rat hepatoma cells, which induces proliferation, also causes a rapid decrease in transcription and expression of the IGFBP-1 gene. Of note, IGFBP-1 mRNA is abundant in fetal rat liver, implying that it participates in normal liver growth and development. Although regenerating liver cells continue to produce IGF-I, we did not detect IGF-I receptor mRNA during the first 24 h after hepatectomy. However, some IGFBPs may act to enhance the activity of IGF-I independently of IGF-I receptors. Thus, IGF-I and IGFBP-1 may interact with hepatocytes or nonparenchymal liver cells, through either IGF-I or novel receptors. In this way, IGFBP-1 and IGF-I could act in a paracrine and/or autocrine fashion in maintaining normal liver architecture during regeneration.
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页码:1393 / 1401
页数:9
相关论文
共 49 条
[1]   COMPLEXITY OF THE EARLY GENETIC RESPONSE TO GROWTH-FACTORS IN MOUSE FIBROBLASTS [J].
ALMENDRAL, JM ;
SOMMER, D ;
MACDONALDBRAVO, H ;
BURCKHARDT, J ;
PERERA, J ;
BRAVO, R .
MOLECULAR AND CELLULAR BIOLOGY, 1988, 8 (05) :2140-2148
[2]  
ARIAS IM, 1988, LIVER BIOL PATHOBIOL, P3
[3]   INSULIN, INSULIN-LIKE GROWTH FACTOR-I AND PLATELET-DERIVED GROWTH-FACTOR INTERACT ADDITIVELY IN THE INDUCTION OF THE PROTOONCOGENE C-MYC AND CELLULAR PROLIFERATION IN CULTURED BOVINE AORTIC SMOOTH-MUSCLE CELLS [J].
BANSKOTA, NK ;
TAUB, R ;
ZELLNER, K ;
KING, GL .
MOLECULAR ENDOCRINOLOGY, 1989, 3 (08) :1183-1190
[4]  
BAR RS, 1990, DIABETES S, V39, pA11
[5]  
BAYNE ML, 1988, J BIOL CHEM, V263, P6233
[6]   A BLOCK TO ELONGATION IS LARGELY RESPONSIBLE FOR DECREASED TRANSCRIPTION OF C-MYC IN DIFFERENTIATED HL60 CELLS [J].
BENTLEY, DL ;
GROUDINE, M .
NATURE, 1986, 321 (6071) :702-706
[7]   HUMAN PROTOONCOGENE C-JUN ENCODES A DNA-BINDING PROTEIN WITH STRUCTURAL AND FUNCTIONAL-PROPERTIES OF TRANSCRIPTION FACTOR AP-1 [J].
BOHMANN, D ;
BOS, TJ ;
ADMON, A ;
NISHIMURA, T ;
VOGT, PK ;
TJIAN, R .
SCIENCE, 1987, 238 (4832) :1386-1392
[8]   CLONING, CHARACTERIZATION, AND EXPRESSION OF A HUMAN INSULIN-LIKE GROWTH-FACTOR BINDING-PROTEIN [J].
BREWER, MT ;
STETLER, GL ;
SQUIRES, CH ;
THOMPSON, RC ;
BUSBY, WH ;
CLEMMONS, DR .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1988, 152 (03) :1289-1297
[9]   ORGANIZATION OF THE GENE ENCODING THE INSULIN-LIKE GROWTH-FACTOR BINDING-PROTEIN IBP-1 [J].
BRINKMAN, A ;
GROFFEN, CAH ;
KORTLEVE, DJ ;
DROP, SLS .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1988, 157 (03) :898-907
[10]   ISOLATION AND CHARACTERIZATION OF A CDNA-ENCODING THE LOW-MOLECULAR WEIGHT INSULIN-LIKE GROWTH-FACTOR BINDING-PROTEIN (IBP-1) [J].
BRINKMAN, A ;
GROFFEN, C ;
KORTLEVE, DJ ;
VANKESSEL, AG ;
DROP, SLS .
EMBO JOURNAL, 1988, 7 (08) :2417-2423