USE OF ELECTROSPRAY MASS-SPECTROMETRY TO INVESTIGATE THE INHIBITION OF BETA-LACTAMASES BY 6-HALOGENOPENICILLANIC ACIDS

被引：12

作者：

APLIN, RT

ROBINSON, CV

SCHOFIELD, CJ

WALEY, SG

机构：

[1] LAB MOLEC BIOPHYS,OXFORD OX1 3QY,ENGLAND

[2] OXFORD CTR MOLEC SCI,OXFORD OX1 3QY,ENGLAND

来源：

JOURNAL OF THE CHEMICAL SOCIETY-CHEMICAL COMMUNICATIONS | 1993年 / 02期

关键词：

D O I：

10.1039/c39930000121

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Electrospray mass spectrometry has been used to investigate the mechanism of inhibition of class A and C beta-lactamases by 6-halogenopenicillanic acids; in the case of the 6beta-halogen substituted penicillanic acids the molecular mass differences observed between the unreacted and the inhibited beta-lactamases are consistent with the formation of an enzyme bound dihydrothiazine derivative, as previously proposed.

引用

页码：121 / 123

页数：3

共 13 条

[1] USE OF ELECTROSPRAY MASS-SPECTROMETRY TO DIRECTLY OBSERVE AN ACYL ENZYME INTERMEDIATE IN BETA-LACTAMASE CATALYSIS
APLIN, RT
BALDWIN, JE
SCHOFIELD, CJ
WALEY, SG
[J]. FEBS LETTERS, 1990, 277 (1-2) : 212 - 214
[2] INACTIVATION OF BACILLUS-CEREUS BETA-LACTAMASE-I BY 6-BETA-BROMOPENICILLANIC ACID - MECHANISM
COHEN, SA
PRATT, RF
[J]. BIOCHEMISTRY, 1980, 19 (17) : 3996 - 4003
[3] SEPARATION, PURIFICATION AND PROPERTIES OF BETA-LACTAMASE I AND BETA-LACTAMASE II FROM BACILLUS-CEREUS 569-H-9
DAVIES, RB
ABRAHAM, EP
MELLING, J
[J]. BIOCHEMICAL JOURNAL, 1974, 143 (01) : 115 - 127
[4] ELECTROSPRAY IONIZATION FOR MASS-SPECTROMETRY OF LARGE BIOMOLECULES
FENN, JB
MANN, M
MENG, CK
WONG, SF
WHITEHOUSE, CM
[J]. SCIENCE, 1989, 246 (4926) : 64 - 71
[5] JORIS B, 1988, BIOCHEM J, V187, P797
[6] 6-BETA-BROMOPENICILLANIC ACID INACTIVATES BETA-LACTAMASE-I
KNOTTHUNZIKER, V
ORLEK, BS
SAMMES, PG
WALEY, SG
[J]. BIOCHEMICAL JOURNAL, 1979, 177 (01) : 365 - 367
[7] INACTIVATION OF BACILLUS-CEREUS BETA-LACTAMASE-I BY 6-BETA-BROMOPENICILLANIC ACID - KINETICS
LOOSEMORE, MJ
COHEN, SA
PRATT, RF
[J]. BIOCHEMISTRY, 1980, 19 (17) : 3990 - 3995
[8] ON THE CHEMISTRY OF BETA-LACTAMASE INHIBITION BY 6-BETA-BROMOPENICILLANIC ACID
ORLEK, BS
SAMMES, PG
KNOTTHUNZIKER, V
WALEY, SG
[J]. JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 1, 1980, (10): : 2322 - 2329
[9] 6-BETA-BROMOPENICILLANIC ACID, A POTENT BETA-LACTAMASE INHIBITOR
PRATT, RF
LOOSEMORE, MJ
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1978, 75 (09) : 4145 - 4149
[10] NEW DEVELOPMENTS IN BIOCHEMICAL MASS-SPECTROMETRY - ELECTROSPRAY IONIZATION
SMITH, RD
LOO, JA
EDMONDS, CG
BARINAGA, CJ
UDSETH, HR
[J]. ANALYTICAL CHEMISTRY, 1990, 62 (09) : 882 - 899

← 1 2 →