INDUCTION OF DNA DOUBLE-STRAND BREAKS IN CHO-K1 CELLS BY CARBON-IONS

被引:46
作者
HEILMANN, J
TAUCHERSCHOLZ, G
KRAFT, G
机构
[1] Gesellschaft für Schwerionenforschung, Postfach 110552, D-64220, Darmstadt
关键词
D O I
10.1080/09553009514551051
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Radiation-induced DNA double-strand breaks (dsbs) were measured in CHO-K1 cells by means of an experimental approach involving constant-field gel electrophoresis and densitometric scanning of ethidium bromide stained gels. For X-irradiation, an induction efficiency of 36 +/- 5 dsbs (Gy X cell)(-1) was determined. With this set-up, the induction of dsbs was investigated in CHO-K1 cells after irradiation with accelerated carbon ions with specific energies ranging from 2.7 to 261 MeV/u. This set of particle beams covers the important linear energy transfer (LET) range between 17 and 400 keV/mu m, where maximum efficiencies have been reported for other cellular endpoints like inactivation or mutation induction. For LETs up to 100 keV/mu m, RBEs of similar to 1 have been determined, while efficiencies per unit dose decline for higher LETs. No RBE maximum > 1 was found. Data are compared with published results on dsb induction in mammalian cells by radiations of comparable LET.
引用
收藏
页码:153 / 162
页数:10
相关论文
共 51 条
[1]   MEASUREMENT OF RADIATION-INDUCED DNA DOUBLE-STRAND BREAKS BY PULSED-FIELD GEL-ELECTROPHORESIS [J].
AGER, DD ;
DEWEY, WC ;
GARDINER, K ;
HARVEY, W ;
JOHNSON, RT ;
WALDREN, CA .
RADIATION RESEARCH, 1990, 122 (02) :181-187
[2]   HEAVY-ION EFFECTS ON CELLULAR DNA - STRAND BREAK INDUCTION AND REPAIR IN CULTURED DIPLOID LENS EPITHELIAL-CELLS [J].
AUFDERHEIDE, E ;
RINK, H ;
HIEBER, L ;
KRAFT, G .
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 1987, 51 (05) :779-790
[3]  
AWA AA, 1974, CHROMOSOMES CANCER
[4]   EFFECTS OF DIFFERENT IONIZING RADIATIONS ON HUMAN CELLS IN TISSUE CULTURE .3. EXPERIMENTS WITH CYCLOTRON-ACCELERATED ALPHA-PARTICLES AND DEUTERONS [J].
BARENDSEN, GW ;
WALTER, HMD ;
BEWLEY, DK ;
FOWLER, JF .
RADIATION RESEARCH, 1963, 18 (01) :106-&
[5]   DNA DOUBLE-STRAND BREAKS INDUCED BY LOW-ENERGY PROTONS IN V79 CELLS [J].
BELLI, M ;
CERA, F ;
CHERUBINI, R ;
IANZINI, F ;
MOSCHINI, G ;
SAPORA, O ;
SIMONE, G ;
TABOCCHINI, MA ;
TIVERON, P .
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 1994, 65 (05) :529-536
[6]   IN CHEF ELECTROPHORESIS A LINEAR INDUCTION OF DSB CORRESPONDS TO A NONLINEAR FRACTION OF EXTRACTED DNA WITH DOSE [J].
BLOCHER, D .
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 1990, 57 (01) :7-12
[7]   CHEF ELECTROPHORESIS, A SENSITIVE TECHNIQUE FOR THE DETERMINATION OF DNA DOUBLE-STRAND BREAKS [J].
BLOCHER, D ;
EINSPENNER, M ;
ZAJACKOWSKI, J .
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 1989, 56 (04) :437-448
[10]   X-RAY-INDUCED DNA DOUBLE STRAND BREAK PRODUCTION AND REPAIR IN MAMMALIAN-CELLS AS MEASURED BY NEUTRAL FILTER ELUTION [J].
BRADLEY, MO ;
KOHN, KW .
NUCLEIC ACIDS RESEARCH, 1979, 7 (03) :793-804