SEQUENCE AND TISSUE-SPECIFIC EXPRESSION OF A PUTATIVE PEROXIDASE GENE FROM WHEAT (TRITICUM-AESTIVUM L)

被引：45

作者：

HERTIG, C ^{[1
]}

REBMANN, G ^{[1
]}

BULL, J ^{[1
]}

MAUCH, F ^{[1
]}

DUDLER, R ^{[1
]}

机构：

[1] UNIV ZURICH,INST PLANT BIOL,ZOLLIKERSTR 107,CH-8008 ZURICH,SWITZERLAND

来源：

PLANT MOLECULAR BIOLOGY | 1991年 / 16卷 / 01期

关键词：

PEROXIDASE GENE; WHEAT;

D O I：

10.1007/BF00017928

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have used a cDNA clone encoding a pathogen-induced putative wheat peroxidase to screen a genomic library of wheat (Triticum aestivum L. cv. Cheyenne) and isolated one positive clone, lambda POX1. Sequence analysis revealed that this clone contains a gene encoding a putative peroxidase with a calculated pI of 8.1 which exhibits 58% and 83% sequence identity to the amino acid sequence of the turnip (Brassica rapa) peroxidase and a pathogen-induced putative wheat peroxidase, respectively. The two introns in the wheat gene are at the same positions as introns in the peroxidase genes of tomato and horseradish. Results of S1-mapping experiments suggest that this gene is neither pathogen- nor wound-induced in leaves but is constitutively expressed in roots.

引用

页码：171 / 174

页数：4

共 5 条

[1] UPSTREAM ELEMENTS NECESSARY FOR OPTIMAL FUNCTION OF THE HSP-70 PROMOTER IN TRANSFORMED FLIES [J].

DUDLER, R ;

TRAVERS, AA .

CELL, 1984, 38 (02) :391-398

[2] STRUCTURE OF THE HORSERADISH-PEROXIDASE ISOZYME-C GENES [J].

FUJIYAMA, K ;

TAKEMURA, H ;

SHIBAYAMA, S ;

KOBAYASHI, K ;

CHOI, JK ;

SHINMYO, A ;

TAKANO, M ;

YAMADA, Y ;

OKADA, H .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1988, 173 (03) :681-687

[3] COVALENT STRUCTURE OF TURNIP PEROXIDASE-7 - CYANOGEN-BROMIDE FRAGMENTS, COMPLETE STRUCTURE AND COMPARISON TO HORSERADISH PEROXIDASE-C [J].

MAZZA, G ;

WELINDER, KG .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1980, 108 (02) :481-489

[4] MOLECULAR-CLONING, NUCLEOTIDE-SEQUENCE, AND ABSCISIC-ACID INDUCTION OF A SUBERIZATION-ASSOCIATED HIGHLY ANIONIC PEROXIDASE [J].

ROBERTS, E ;

KOLATTUKUDY, PE .

MOLECULAR & GENERAL GENETICS, 1989, 217 (2-3) :223-232

[5] CDNA CLONING, INVITRO TRANSCRIPTION AND PARTIAL SEQUENCE-ANALYSIS OF MESSENGER-RNAS FROM WINTER-WHEAT (TRITICUM-AESTIVUM L) WITH INDUCED RESISTANCE TO ERYSIPHE-GRAMINIS F-SP TRITICI [J].

SCHWEIZER, P ;

HUNZIKER, W ;

MOSINGER, E .

PLANT MOLECULAR BIOLOGY, 1989, 12 (06) :643-654

← 1 →