NUCLEOTIDE-SEQUENCE OF A NOVEL DELTA-ENDOTOXIN GENE CRYIG OF BACILLUS-THURINGIENSIS SSP GALLERIAE

被引:22
作者
SMULEVITCH, SV [1 ]
OSTERMAN, AL [1 ]
SHEVELEV, AB [1 ]
KALUGER, SV [1 ]
KARASIN, AI [1 ]
KADYROV, RM [1 ]
ZAGNITKO, OP [1 ]
CHESTUKHINA, GG [1 ]
STEPANOV, VM [1 ]
机构
[1] MOSCOW MICROBIAL GENET INST,PROT CHEM LAB,1ST DOROZHNY 1,MOSCOW 113545,USSR
关键词
CRYIG; DELTA-ENDOTOXIN; PRIMARY STRUCTURE; GENOMIC LIBRARY; BACILLUS-THURINGIENSIS;
D O I
10.1016/0014-5793(91)81144-W
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A gene cryIg coding for entomocidal protein delta-endotoxin of Bacillus thuringiensis ssp. galleriae str. 11-67 named CryIG has been cloned and sequenced (EMBL accession number X58120). The deduced amino acid sequence that contains 1156 amino acid residues shows only 28% of identical residues, when compared with other delta-endotoxins of the CryI family. The extent of identity is substantially higher for some regions of the sequence conserved blocks'), that presumably bear important structural or functional properties. This implies that CryIG delta-endotoxin follows the same type of polypeptide chain folding as other CryI proteins, whereas peculiarities of primary structure help to explain its unique specificity.
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收藏
页码:25 / 28
页数:4
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