EFFECTIVE INDUCTION OF NEUTRALIZING ANTIBODIES WITH THE AMINO-TERMINUS OF VP2 OF CANINE PARVOVIRUS AS A SYNTHETIC PEPTIDE

被引:50
作者
LANGEVELD, JPM
CASAL, JI
CORTES, E
VANDEWETERING, G
BOSHUIZEN, RS
SCHAAPER, WMM
DALSGAARD, K
MELOEN, RH
机构
[1] IMMUNOL & GENET APLICADA SA,E-28037 MADRID,SPAIN
[2] STATE VET INST VIRUS RES,DK-4771 KALVEHAVE,DENMARK
关键词
PARVOVIRUS; PEPTIDE VACCINE; NEUTRALIZING ANTIBODIES;
D O I
10.1016/0264-410X(94)90158-9
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Fourteen synthetic peptides corresponding to previously wrapped antigenic sites in VP2 of canine parvovirus (CPV) were used for immunization of rabbits to identify antiviral properties favourable for inclusion into a vaccine. Most antipeptide antisera obtained were reactive with viral protein, and with one of them it was possible to locate the hypothetical amino terminus of VP3 within positions 15-31 of VP2. Virus-neutralizing antibodies were only obtained with two overlapping 15-mer peptides corresponding in sequence to the amino terminus of VP2 (MSDGAVQPDGGQPAVRNERAT). Antibodies in the neutralizing sera bound most strongly to amino acids of the sequence DGGQPAV within the N-terminus of VP2, indicating that efforts to develop a synthetic vaccine against CVP should be focused on this stretch of amino acids. The two peptides induced long-lasting immunity (at least 8 months) using either Freund's adjuvant or aluminium hydroxide plus Quil A. Thus, this approach delineated the exact peptide sequence useful for vaccines applied to the amino-terminal region of VP2. These findings in experimental animals form a solid basis for exploration of a synthetic peptide vaccine against parvovirus infection in dogs, minks or cats.
引用
收藏
页码:1473 / 1480
页数:8
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