DETERMINATION OF CALCIUM CONTENT IN INDIVIDUAL BIOLOGICAL CELLS BY INDUCTIVELY-COUPLED PLASMA-ATOMIC EMISSION-SPECTROMETRY

被引：58

作者：

NOMIZU, T ^{[1
]}

KANECO, S ^{[1
]}

TANAKA, T ^{[1
]}

ITO, D ^{[1
]}

KAWAGUCHI, H ^{[1
]}

VALLEE, BT ^{[1
]}

机构：

[1] HARVARD UNIV,SCH MED,CTR BIOCHEM & BIOPHYS SCI & MED,BOSTON,MA 02115

来源：

ANALYTICAL CHEMISTRY | 1994年 / 66卷 / 19期

关键词：

D O I：

10.1021/ac00091a004

中图分类号：

O65 [分析化学];

学科分类号：

070302 [分析化学]; 081704 [应用化学];

摘要：

A cell analyzer is described which can continuously determine the calcium content in individual biological cells by inductively coupled plasma atomic emission spectrometry in real time. A cell suspension in ethanol (70% in water) is sprayed by a concentric nebulizer, and the droplets containing cells are dried in a drying chamber. The dried cells dispersed in air are directly introduced into the plasma to determine the calcium by emission spectrometry. Each flash derived from each cell in the plasma is converted into an electric pulse corresponding to the calcium content in the cell, and the heights of the pulses are measured by a pulse height analyzer. To calibrate this system, monodisperse calcium acetate aerosols are prepared by a vibrating orifice monodisperse aerosol generator. The determined calcium contents in several mammalian cell samples range from 0.057 to 0.27 pg. The detection limit of the present system is similar to 0.01 pg of calcium in a cell.

引用

页码：3000 / 3004

页数：5

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