Cloning and expression of canine interleukin-10

被引：12

作者：

Lu, P

Zucker, K

Fuller, L

Tzakis, A

Esquenazi, V

Miller, J

机构：

[1] UNIV MIAMI, SCH MED, DEPT MICROBIOL & IMMUNOL, MIAMI, FL 33101 USA

[2] UNIV MIAMI, SCH MED, DEPT MED, MIAMI, FL 33101 USA

[3] UNIV MIAMI, SCH MED, DEPT PATHOL, MIAMI, FL 33101 USA

[4] MIAMI VET ADMIN HOSP, MIAMI, FL 33101 USA

来源：

JOURNAL OF INTERFERON AND CYTOKINE RESEARCH | 1995年 / 15卷 / 12期

关键词：

D O I：

10.1089/jir.1995.15.1103

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe here the cloning of canine IL-10 cDNA (GenBank accession No, U33843) and the expression of recombinant IL-10 in a dog kidney cell line (DK6247) and Chinese hamster ovary cells (CHO). Canine IL-10 exhibits strong sequence homology to the known sequences of human, mouse, rat, and bovine genes at nucleotide and amino acid levels. The IL-10 gene, when introduced into DK and CHO cell lines, produces recombinant IL-10 that causes an inhibitory effect on allogeneic MHC-driven lymphoproliferative responses.

引用

页码：1103 / 1109

页数：7

共 17 条

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