CLINICAL ANALYSIS IN INTACT ERYTHROCYTES USING H-1 SPIN-ECHO NMR

被引:23
作者
REGLINSKI, J
SMITH, WE
WILSON, R
BUCHANAN, LM
MCKILLOP, JH
THOMSON, JA
BRZESKI, M
MARABANI, M
STURROCK, RD
机构
[1] UNIV GLASGOW,DEPT MED,GLASGOW G12 8QQ,SCOTLAND
[2] GLASGOW ROYAL INFIRM,CTR RHEUMAT DIS,GLASGOW G4 0SF,SCOTLAND
关键词
ERYTHROCYTE; CLINICAL ANALYSIS; RHEUMATOID ARTHRITIS; GRAVES DISEASE; GLUTATHIONE;
D O I
10.1016/0009-8981(91)90023-6
中图分类号
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号
1001 ;
摘要
A new method of clinical analysis based on H-1 spin echo NMR spectroscopy is presented. It is capable of providing information on six metabolites within viable erythrocytes, directly and without any preparative procedures prior to analysis except for cell separation and washing. Erythrocytes from patients with rheumatoid arthritis and Graves' disease are compared with cells obtained from healthy volunteers. The NMR detectable species in the cytosol of the cells are glutathione, ergothioneine, choline, creatine, glycine, lactate and to a lesser extent alanine and valine. Significant differences are observed between the ergothioneine pools in the rheumatoid group (P < 0.01) compared to the control group. The glutathione: di-glutathione ratio can be assessed from the ratio, g2 to g4, taken from different signals in the glutathione molecule. The total concentration of glutathione present is easily assessed qualitatively but is more difficult to quantitate.
引用
收藏
页码:45 / 58
页数:14
相关论文
共 19 条
  • [1] THE USE OF H-1 SPIN-ECHO NMR AND HPLC TO CONFIRM DOXORUBICIN INDUCED DEPLETION OF GLUTATHIONE IN THE INTACT HELA-CELL
    ALKABBAN, M
    WATSON, ID
    STEWART, MJ
    REGLINSKI, J
    SMITH, WE
    SUCKLING, CJ
    [J]. BRITISH JOURNAL OF CANCER, 1988, 57 (06) : 553 - 558
  • [2] THE AMERICAN-RHEUMATISM-ASSOCIATION 1987 REVISED CRITERIA FOR THE CLASSIFICATION OF RHEUMATOID-ARTHRITIS
    ARNETT, FC
    EDWORTHY, SM
    BLOCH, DA
    MCSHANE, DJ
    FRIES, JF
    COOPER, NS
    HEALEY, LA
    KAPLAN, SR
    LIANG, MH
    LUTHRA, HS
    MEDSGER, TA
    MITCHELL, DM
    NEUSTADT, DH
    PINALS, RS
    SCHALLER, JG
    SHARP, JT
    WILDER, RL
    HUNDER, GG
    [J]. ARTHRITIS AND RHEUMATISM, 1988, 31 (03): : 315 - 324
  • [3] DETERMINATION OF THIOL CONCENTRATIONS IN HEMOLYSATE BY RESONANCE RAMAN SPECTROMETRY
    BANFORD, JC
    BROWN, DH
    MCCONNELL, AA
    MCNEILL, CJ
    SMITH, WE
    HAZELTON, RA
    STURROCK, RD
    [J]. ANALYST, 1982, 107 (1271) : 195 - 199
  • [4] ALTERED THIOL STATUS IN PATIENTS WITH RHEUMATOID-ARTHRITIS
    BANFORD, JC
    BROWN, DH
    HAZELTON, RA
    MCNEIL, CJ
    SMITH, WE
    STURROCK, RD
    [J]. RHEUMATOLOGY INTERNATIONAL, 1982, 2 (03) : 107 - 111
  • [5] APPLICATION OF SPIN-ECHO NUCLEAR MAGNETIC-RESONANCE TO WHOLE-CELL SYSTEMS - MEMBRANE-TRANSPORT
    BRINDLE, KM
    BROWN, FF
    CAMPBELL, ID
    GRATHWOHL, C
    KUCHEL, PW
    [J]. BIOCHEMICAL JOURNAL, 1979, 180 (01) : 37 - 44
  • [6] HUMAN ERYTHROCYTE METABOLISM STUDIES BY H-1 SPIN-ECHO NMR
    BROWN, FF
    CAMPBELL, ID
    KUCHEL, PW
    RABENSTEIN, DC
    [J]. FEBS LETTERS, 1977, 82 (01) : 12 - 16
  • [7] NMR-STUDIES OF RED-CELLS
    BROWN, FF
    CAMPBELL, ID
    [J]. PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY OF LONDON SERIES B-BIOLOGICAL SCIENCES, 1980, 289 (1037) : 395 - 406
  • [8] BRZESKI M, 1988, British Journal of Rheumatology, V27, P26
  • [9] THE OXIDATION OF GLUTATHIONE BY NITROPRUSSIDE - CHANGES IN GLUTATHIONE IN INTACT ERYTHROCYTES DURING INCUBATION WITH SODIUM-NITROPRUSSIDE AS DETECTED BY H-1 SPIN-ECHO NMR-SPECTROSCOPY
    BUTLER, AR
    CALSYHARRISON, AM
    GLIDEWELL, C
    JOHNSON, IL
    REGLINSKI, J
    SMITH, WE
    [J]. INORGANICA CHIMICA ACTA-BIOINORGANIC CHEMISTRY, 1988, 151 (04): : 281 - 286
  • [10] CHANGES IN GLUTATHIONE IN INTACT ERYTHROCYTES DURING INCUBATION WITH PENICILLAMINE AS DETECTED BY H-1 SPIN-ECHO NMR-SPECTROSCOPY
    MCKAY, CNN
    BROWN, DH
    REGLINSKI, J
    SMITH, WE
    CAPELL, HA
    STURROCK, RD
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1986, 888 (01) : 30 - 35