CONSTRUCTION AND CHARACTERIZATION OF A PARTIAL LIBRARY OF YEAST ARTIFICIAL CHROMOSOMES FROM HUMAN CHROMOSOME-21

被引：14

作者：

BELLIS, M

GERARD, A

CHARLIEU, JP

MARCAIS, B

BRUN, ME

VIEGASPEQUIGNOT, E

CARTER, DA

ROIZES, G

机构：

[1] INST BIOL, INSERM, U249, F-34060 MONTPELLIER, FRANCE

[2] INST CURIE, CNRS, URA 620, F-675231 PARIS 05, FRANCE

来源：

DNA AND CELL BIOLOGY | 1991年 / 10卷 / 04期

关键词：

D O I：

10.1089/dna.1991.10.301

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report a protocol for cloning large DNA fragments in yeast artificial chromosomes (YAC). A partial library has been constructed from a somatic hybrid containing chromosome 21 as the single source of human DNA. About 4.0 Mb of human DNA was recovered in 17 YAC clones. Three clones were analyzed by in situ hybridization and mapped on chromosome 21. One clone hybridized with the chromosome 21 centromeric region and may provide new insight both on the molecular structure of centromere and on the localization of Alzheimer disease gene.

引用

页码：301 / 310

页数：10

共 64 条

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