REPORTER CONSTRUCTS WITH LOW BACKGROUND ACTIVITY UTILIZING THE CAT GENE

被引:250
作者
BOSHART, M [1 ]
KLUPPEL, M [1 ]
SCHMIDT, A [1 ]
SCHUTZ, G [1 ]
LUCKOW, B [1 ]
机构
[1] GERMAN CANC RES CTR,INST CELL & TUMOR BIOL,IM NEUENHEIMER FELD 280,W-6900 HEIDELBERG 1,GERMANY
关键词
RECOMBINANT DNA; CHLORAMPHENICOL ACETYLTRANSFERASE ASSAY; GENE TRANSFER; PROMOTER ENHANCER ANALYSIS; TRANSCRIPTIONAL REGULATION; CRYPTIC PROMOTER ENHANCER;
D O I
10.1016/0378-1119(92)90456-Y
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Reporter plasmids utilizing the cat gene for the analysis of promoter and enhancer sequences in vertebrate cells, were constructed. These plasmids minimize the background of transcription derived from cryptic promoters or cryptic regulatory elements within the vector.
引用
收藏
页码:129 / 130
页数:2
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