TRANSIENT GENE-EXPRESSION IN STRAWBERRY (FRAGARIA X ANANASSA DUCH) PROTOPLASTS AND THE RECOVERY OF TRANSGENIC PLANTS

被引：83

作者：

NYMAN, M

WALLIN, A

机构：

来源：

PLANT CELL REPORTS | 1992年 / 11卷 / 02期

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D O I：

暂无

中图分类号：

Q94 [植物学];

学科分类号：

071001 [植物学];

摘要：

A transient beta-glucuronidase (GUS)-assay was performed to evaluate electroporation parameters and optimize DNA delivery conditions into strawberry protoplasts. Optimal GUS-activity was obtained when protoplasts were subjected to 400 V/cm for 20 ms. GUS-activity could be further increased by the addition of carrier DNA to the electroporation mixture. Callus selected on 10-mu-g/ml hygromycin produced shoots which exhibited GUS-activity. The transformed nature of the shoots obtained after selection was confirmed by DNA-analysis.

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页码：105 / 108

页数：4

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