CHARACTERIZATION OF INTERLEUKIN-1 PRODUCTION BY MICROGLIA IN CULTURE

被引：117

作者：

YAO, JB

KERI, JE

TAFFS, RE

COLTON, CA

机构：

[1] GEORGETOWN UNIV, SCH MED, DEPT PHYSIOL & BIOPHYS, WASHINGTON, DC 20007 USA

[2] NINCDS, BIOPHYS LAB, BETHESDA, MD 20892 USA

[3] US FDA, CTR BIOL EVALUAT & RES, BETHESDA, MD 20014 USA

来源：

BRAIN RESEARCH | 1992年 / 591卷 / 01期

关键词：

MICROGLIA; MACROPHAGE; INTERLEUKIN-1; LIPOPOLYSACCHARIDE;

D O I：

10.1016/0006-8993(92)90981-E

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The production of interleukin-1 (IL-1) by cultured neonatal rat microglia was studied using the D10 cell assay. The results show that IL-1 was secreted in response to lipopolysaccharide (LPS) in a dose- and time-dependent fashion. IL-1 production was specific to microglia and was not induced in astrocytes. Indomethacin, which is known to modulate the release of IL-1 from monocytes, had no effect on LPS-stimulated microglia. Aging of the microglia from two weeks to 4 weeks in culture, however, reduced the release of IL-1 in response to LPS. Our data indicate that microglia are a major source of IL-1 and that the release of IL-1 depends on the presence of inflammatory mediators such as LPS and the age of the culture.

引用

页码：88 / 93

页数：6

共 37 条

[1] MAJOR HISTOCOMPATIBILITY COMPLEX ANTIGEN EXPRESSION ON RAT MICROGLIA FOLLOWING EPIDURAL KAINIC ACID LESIONS [J].