MOLECULAR-CLONING OF A NOVEL RAT G-PROTEIN-COUPLED RECEPTOR GENE EXPRESSED PROMINENTLY IN LUNG, ADRENAL, AND LIVER

被引：25

作者：

HARRISON, JK

BARBER, CM

LYNCH, KR

机构：

[1] UNIV VIRGINIA,SCH MED,DEPT PHARMACOL,BOX 448,1300 JEFFERSON PK AVE,CHARLOTTESVILLE,VA 22908

[2] UNIV VIRGINIA,SCH MED,DEPT BIOCHEM,CHARLOTTESVILLE,VA 22908

来源：

FEBS LETTERS | 1993年 / 318卷 / 01期

关键词：

G-PROTEIN-COUPLED RECEPTOR; PUTATIVE G-PROTEIN-COUPLED RECEPTOR; ORPHAN RECEPTOR; RHODOPSIN FAMILY; GCR;

D O I：

10.1016/0014-5793(93)81318-T

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A gene and cDNA (named G10d) encoding a novel member of the G-protein-coupled receptor (GCR) superfamily has been isolated from a rat genomic library and a rat liver cDNA library by tandom polymerase chain reaction (PCR) amplification and hybridization screening. Comparison of the sequence of the encoded protein to other members of the rhodopsin family of the GCR superfamily suggests that this new member is a peptide receptor. The G10d gene is present as a single copy in the rat genome and its translational open reading frame is intronless. Northern analysis demonstrates a wide and uneven distribution of G10d mRNA in the adult rat, with the highest mRNA abundance in lung, liver and adrenal gland extracts.

引用

页码：17 / 22

页数：6

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