HPLC OF SYNTHETIC-POLYMERS CHARACTERIZATION OF POLYSTYRENES BY HIGH-PERFORMANCE PRECIPITATION LIQUID-CHROMATOGRAPHY (HPPLC)

被引:28
作者
SCHULTZ, R [1 ]
ENGELHARDT, H [1 ]
机构
[1] UNIV SAARLAND,W-6600 SAARBRUCKEN,GERMANY
关键词
Colloidal elution; Elution conditions; High performance precipitation liquid chromagraphy; Non-porous stationary phases; Polystyrenes;
D O I
10.1007/BF02317905
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The advantages and disadvantages of high performance precipitation liquid chromatography have been demonstrated for polystyrene homopolymers. Depending on the mobile phase composition at the dissolution point of the polymeric sample and surface properties of the stationary phase, elution is governed either by a solution process or by adsorption. A contribution by adsorption was noticed on silica as well as on reversed phases based on silica with a normal phase gradient of increasing polarity (heptane to dichloromethane). Elution was solely governed by solubility of the polymers on both types of stationary phase for polystyrenes with a molecular weight above 35 000 and reversed phase gradient of decreasing polarity (methanol to dichloromethane). Under these conditions an identical dependence of elution solvent composition on sample size was found as for turbidity titrations. Due to differences in the velocity of the eluent front and the polymeric sample with porous stationary phases the polymers can be eluted as colloidal solutions Non-porous stationary phases are superior in this respect because the velocities of eluent and solutes are identical. © 1990 Friedr. Vieweg & Sohn Verlagsgesellschaft mbH.
引用
收藏
页码:205 / 213
页数:9
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