EFFECT OF MONOMETHYLETHANOLAMINE, DIMETHYLETHANOLAMINE, GANGLIOSIDES, ISOPROTERENOL, AND 2-HYDROXYETHYLHYDRAZINE ON THE CONVERSION OF ETHANOLAMINE TO METHYLATED PRODUCTS BY CULTURED CHICK BRAIN NEURONS

被引:12
作者
ANDRIAMAMPANDRY, C
FREYSZ, L
KANFER, JN
DREYFUS, H
MASSARELLI, R
机构
[1] CNRS,INSERM,U44,CTR NEUROCHIM,ANTENNE CRONENBOURG 23 RUE DE LOESS,BP 20CR,F-67037 STRASBOURG,FRANCE
[2] UNIV MANITOBA,DEPT BIOCHEM,WINNIPEG R3T 2N2,MANITOBA,CANADA
关键词
D O I
10.1111/j.1471-4159.1991.tb03439.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The sequential methylation of ethanolamine and its phosphorylated derivatives has been studied with chick neurons in culture in the presence of several pharmacological agents. Incubation with [H-3]ethanolamine in the presence of monomethylethanolamine and dimethylethanolamine indicated that in these neurons the preferential conversion to choline-containing compounds is via the methylation of phosphorylethanolamine. The possibility that there are two separate enzymes, i.e., one responsible for the methylation of water-soluble ethanolamine-containing compounds and another for the ethanolamine phospholipids, was examined with agents believed to influence these conversions. Incubation of neurons in the presence of a mixture of exogenous gangliosides at 10(-8) M and 10(-5) M concentrations showed that these neuritogenic compounds stimulate the methylation of phosphatidylethanolamine and decrease that of phosphorylethanolamine. The inhibitor of phosphatidylethanolamine methyltransferase (EC 2.1.1.17), 2-hydroxyethylhydrazine, decreased the conversion of phosphatidylethanolamine to phosphatidylcholine and increased that of phosphorylethanolamine to phosphorylcholine. The possible effects of adrenergic stimulation were studied by the incubation of neurons with isoproterenol at 10(-6) M and 10(-5) M concentrations. There was a reduction of phosphorylethanolamine methylation and a stimulation of that of phosphatidylethanolamine, and these effects were counteracted by the presence of 5 x 10(-5) M propranolol.
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页码:1845 / 1850
页数:6
相关论文
共 27 条
[1]   A RAT-BRAIN CYTOSOLIC N-METHYLTRANSFERASE(S) ACTIVITY CONVERTING PHOSPHORYLETHANOLAMINE INTO PHOSPHORYLCHOLINE [J].
ANDRIAMAMPANDRY, C ;
MASSARELLI, R ;
FREYSZ, L ;
KANFER, JN .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1990, 171 (02) :758-763
[2]   CONVERSION OF ETHANOLAMINE, MONOMETHYLETHANOLAMINE AND DIMETHYLETHANOLAMINE TO CHOLINE-CONTAINING COMPOUNDS BY NEURONS IN CULTURE AND BY THE RAT-BRAIN [J].
ANDRIAMAMPANDRY, C ;
FREYSZ, L ;
KANFER, JN ;
DREYFUS, H ;
MASSARELLI, R .
BIOCHEMICAL JOURNAL, 1989, 264 (02) :555-562
[3]  
AUDUBERT F, 1983, J BIOL CHEM, V258, P695
[4]   CHEMICAL METHYLATION OF PHOSPHATIDYLETHANOLAMINE BY S-ADENOSYLMETHIONINE [J].
BANSAL, VS ;
KANFER, JN .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1985, 128 (01) :411-416
[5]   IDENTIFICATION AND PROPERTIES OF METHYLTRANSFERASES THAT SYNTHESIZE PHOSPHATIDYLCHOLINE IN RAT-BRAIN SYNAPTOSOMES [J].
CREWS, FT ;
HIRATA, F ;
AXELROD, J .
JOURNAL OF NEUROCHEMISTRY, 1980, 34 (06) :1491-1498
[6]   SYNTHESIS OF CHOLINE PHOSPHOLIPIDS IN NEURONAL AND GLIAL-CELL CULTURES BY THE METHYLATION PATHWAY [J].
DAINOUS, F ;
FREYSZ, L ;
MOZZI, R ;
DREYFUS, H ;
LOUIS, JC ;
PORCELLATI, G ;
MASSARELLI, R .
FEBS LETTERS, 1982, 146 (01) :221-223
[7]   THE INCORPORATION OF MONOMETHYLETHANOLAMINE AND DIMETHYLETHANOLAMINE IN FETAL BRAIN AGGREGATING CELL-CULTURE [J].
DAINOUS, F ;
KANFER, JN .
NEUROCHEMICAL RESEARCH, 1988, 13 (01) :1-8
[8]   PROMOTION OF NEURITOGENESIS IN MOUSE NEURO-BLASTOMA CELLS BY EXOGENOUS GANGLIOSIDES - RELATIONSHIP BETWEEN THE EFFECT AND THE CELL ASSOCIATION OF GANGLIOSIDE GM1 [J].
FACCI, L ;
LEON, A ;
TOFFANO, G ;
SONNINO, S ;
GHIDONI, R ;
TETTAMANTI, G .
JOURNAL OF NEUROCHEMISTRY, 1984, 42 (02) :299-305
[9]  
FOLCH J, 1957, J BIOL CHEM, V226, P497
[10]  
GIL MG, 1980, BIOCHEM BIOPH RES CO, V95, P1325