HIV-INFECTION LEADS TO DIFFERENTIAL EXPRESSION OF T-CELL RECEPTOR V-BETA GENES IN CD4+ AND CD8+ T-CELLS

被引:50
作者
HODARA, VL
JEDDITEHRANI, M
GRUNEWALD, J
ANDERSSON, R
SCARLATTI, G
ESIN, S
HOLMBERG, V
LIBONATTI, O
WIGZELL, H
机构
[1] SODER SJUKHUSET,STOCKHOLM,SWEDEN
[2] UNIV BUENOS AIRES,SCH MED,DEPT MICROBIOL,BUENOS AIRES,ARGENTINA
[3] KAROLINSKA INST,DEPT VIROL,S-10401 STOCKHOLM 60,SWEDEN
关键词
T-CELL RECEPTOR; T-CELLS; POLYMERASE CHAIN REACTION; HIV; SUPERANTIGENS;
D O I
10.1097/00002030-199305000-00004
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Objective: To analyse variation in T-cell receptor (TCR) Vbeta gene expression in T cells in HIV-infected individuals. Design: Because there are very few monoclonal antibodies available for studying TCR Vbeta gene expression, we used polymerase chain reaction (PCR) to analyse the TCR Vbeta repertoire in HIV-infected individuals using specific primers for 20 distinct families of TCR Vbeta. Methods: Evaluation of TCR Vbeta gene expression in peripheral blood from HIV-1-infected individuals [two in Centers for Disease Control (CDC) stage II, five in CDC stage III and four in CDC stage IV]. Complementary DNA was produced from CD4+ and CD8+ T cells, amplified by PCR and analysed after Southern blotting and hybridization with a Cbeta-specific oligonucleotide probe. Results: Vbeta gene expression was dramatically modified, especially in AIDS patients. The CD4+ T-cell subset showed both overexpression (Vbeta2) and deletions or underexpression (Vbeta9-Vbeta20), whereas these gene segments were expressed normally in the CD8+ subset. Only Vbeta 3 was deleted or underexpression in both CD4+ and CD8+ populations in AIDS patients. Conclusions: HIV-1 infection induces CD4+ T-cell deficiency, both in total numbers and by causing a paucity of select Vbeta gene expression in this subset. In addition, the Vbeta3 gene family was deleted or underexpressed was observed in both CD4+ and CD8+ T-cell subsets from patients in CDC stage IV. These results are compatible with changes in Vbeta gene expression known to occur under the action of endogenous or exogenous superantigens.
引用
收藏
页码:633 / 638
页数:6
相关论文
共 33 条
  • [1] ASHER MS, 1990, J ACQ IMMUN DEF SYND, V3, P177
  • [2] ASHER MS, 1988, CLIN EXP IMMUNOL, V73, P165
  • [3] GENOMICALLY IMPOSED AND SOMATICALLY MODIFIED HUMAN THYMOCYTE V-BETA GENE REPERTOIRES
    BACCALA, R
    KONO, DH
    WALKER, S
    BALDERAS, RS
    THEOFILOPOULOS, AN
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (07) : 2908 - 2912
  • [4] T-CELLS, V-GENES, AND HIV
    BANSAL, AS
    GREEN, LM
    PUMPHREY, RSH
    MANDAL, B
    [J]. LANCET, 1992, 339 (8809) : 1604 - 1604
  • [5] BRINCHMAN JE, 1990, J VIROL, V65, P2019
  • [6] BYRNE JA, 1988, J IMMUNOL, V141, P3249
  • [7] SUPERANTIGENS
    CHATILA, T
    GEHA, RS
    [J]. CURRENT OPINION IN IMMUNOLOGY, 1992, 4 (01) : 74 - 78
  • [8] INTERACTION OF STAPHYLOCOCCUS-AUREUS TOXIN SUPERANTIGENS WITH HUMAN T-CELLS
    CHOI, YW
    KOTZIN, B
    HERRON, L
    CALLAHAN, J
    MARRACK, P
    KAPPLER, J
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (22) : 8941 - 8945
  • [9] T-CELL RECEPTOR VARIABLE GENE-PRODUCTS AND EARLY HIV-1 INFECTION
    DALGLEISH, AG
    WILSON, S
    GOMPELS, M
    LUDLAM, C
    GAZZARD, B
    COATES, AM
    HABESHAW, J
    [J]. LANCET, 1992, 339 (8797) : 824 - 828
  • [10] T-CELL RECEPTOR VARIABLE BETA-GENES SHOW DIFFERENTIAL EXPRESSION IN CD4 AND CD8 T-CELLS
    DAVEY, MP
    MEYER, MM
    MUNKIRS, DD
    BABCOCK, D
    BRAUN, MP
    HAYDEN, JB
    BAKKE, AC
    [J]. HUMAN IMMUNOLOGY, 1991, 32 (03) : 194 - 202