APPLICATION OF A COMPARATIVE-EVALUATION OF SEVERAL REVERSED-PHASE COLUMNS TO THE AUTOMATED-ANALYSIS OF CANDIDATE PHARMACEUTICALS

被引：21

作者：

MUTTON, IM

机构：

[1] Structural Chemistry Department, Glaxo Research and Development Ltd., Greenford, Middlesex UB6 0HE, Greenford Road

来源：

JOURNAL OF CHROMATOGRAPHY A | 1995年 / 697卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(94)01219-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This report discusses the evaluation of several modern reversed-phase chromatographic packing materials, and describes the application of a selected column in a method used to screen the purities of pharmaceutical candidates and intermediates. Columns are first evaluated with an acetonitrile gradient using a test mixture containing acids, bases and compounds of widely differing polarities. Selected phases are further assessed by analysing many typical samples. Finally, the use of the chosen column and method are described and discussed.

引用

页码：191 / 201

页数：11