EXPRESSION OF THE HYGROMYCIN-B PHOSPHOTRANSFERASE GENE CONFERS TOLERANCE TO THE HERBICIDE GLYPHOSATE

被引：4

作者：

PENALOZAVAZQUEZ, A ^{[1
]}

OROPEZA, A ^{[1
]}

MENA, GL ^{[1
]}

BAILEY, AM ^{[1
]}

机构：

[1] IPN,CINVESTAV,UNIDAD IRAPUATO,DEPT INGN GENET PLANTAS,IRAPUATO 36500,GUANAJUATO,MEXICO

来源：

PLANT CELL REPORTS | 1995年 / 14卷 / 08期

关键词：

D O I：

暂无

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Escherichia coli cells and tobacco (cv. Xanthi) plants transformed with the hygromycin B phosphotransferase gene were able to grow in culture medium containing glyphosate at 2.0 mM. The growth of tobacco calli in media containing increasing glyphosate concentrations was measured. The ID50 for glyphosate was 1.70+/-0.03 mM for hygromycin-B resistant plants, and 0.45+/-0.02 mM for control plants. Regenerated plants and progeny selected for resistance to hygromycin B were tested for glyphosate tolerance by spraying them with Faena herbicide (formulated glyphosate with surfactant) at a dose equal to 0.24 kg/ha. This was two times the dose required to kill 100 percent of the control plants. Phosphotransferase activity was measured in the extracts of the transformed leaves by the incorporation of P-32 from [gamma(32)P]ATP and it was observed that hygromycin B phosphotransferase was able to recognize the molecule of glyphosate as substrate.

引用

页码：482 / 487

页数：6

共 37 条

[1] INHERITANCE OF 2 MUTATIONS CONFERRING GLYPHOSATE TOLERANCE IN THE FERN CERATOPTERIS-RICHARDII [J].

CHUN, PT ;

HICKOK, LG .

CANADIAN JOURNAL OF BOTANY-REVUE CANADIENNE DE BOTANIQUE, 1992, 70 (05) :1097-1099

[2] EXPRESSION IN PLANTS OF A MUTANT AROA GENE FROM SALMONELLA-TYPHIMURIUM CONFERS TOLERANCE TO GLYPHOSATE [J].

COMAI, L ;

FACCIOTTI, D ;

HIATT, WR ;

THOMPSON, G ;

ROSE, RE ;

STALKER, DM .

NATURE, 1985, 317 (6039) :741-744

[3] MOLECULAR APPROACHES TO THE STUDY OF AMINO-ACID BIOSYNTHESIS IN PLANTS [J].

CORUZZI, GM .

PLANT SCIENCE, 1991, 74 (02) :145-155

[4]

DEBLAERE R, 1985, NUCLEIC ACIDS RES, V13, P4777, DOI 10.1093/nar/13.13.4777

[5] TARGETING A HERBICIDE-RESISTANT ENZYME FROM ESCHERICHIA-COLI TO CHLOROPLASTS OF HIGHER-PLANTS [J].

DELLACIOPPA, G ;

BAUER, SC ;

TAYLOR, ML ;

ROCHESTER, DE ;

KLEIN, BK ;

SHAH, DM ;

FRALEY, RT ;

KISHORE, GM .

BIO-TECHNOLOGY, 1987, 5 (06) :579-584

[6]

Dellaporta S.L., 1983, PLANT MOL BIOL REP, V1, P19, DOI [10.1007/BF02712670, DOI 10.1007/BF02712670]

[7] GLYPHOSATE TOLERANCE IN TOBACCO (NICOTIANA-TABACUM-L) [J].

DYER, WE ;

WELLER, SC ;

BRESSAN, RA ;

HERRMANN, KM .

PLANT PHYSIOLOGY, 1988, 88 (03) :661-666

[8] A TECHNIQUE FOR RADIOLABELING DNA RESTRICTION ENDONUCLEASE FRAGMENTS TO HIGH SPECIFIC ACTIVITY [J].

FEINBERG, AP ;

VOGELSTEIN, B .

ANALYTICAL BIOCHEMISTRY, 1983, 132 (01) :6-13

[9] EFFICIENT TRANSFER OF A GLYPHOSATE TOLERANCE GENE INTO TOMATO USING A BINARY AGROBACTERIUM-TUMEFACIENS VECTOR [J].

FILLATTI, JJ ;

KISER, J ;

ROSE, R ;

COMAI, L .

BIO-TECHNOLOGY, 1987, 5 (07) :726-730

[10] CLONING OF A GENE FROM PSEUDOMONAS SP STRAIN PG2982 CONFERRING INCREASED GLYPHOSATE RESISTANCE [J].

FITZGIBBON, JE ;

BRAYMER, HD .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1990, 56 (11) :3382-3388

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