INDUCIBLE AND CONSTITUTIVE EXPRESSION OF VANC-1-ENCODED RESISTANCE TO VANCOMYCIN IN ENTEROCOCCUS-GALLINARUM

被引:44
作者
SAHM, DF [1 ]
FREE, L [1 ]
HANDWERGER, S [1 ]
机构
[1] ROCKEFELLER UNIV,MICROBIOL LAB,NEW YORK,NY 10021
关键词
D O I
10.1128/AAC.39.7.1480
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Clinical isolates Enterococcus gallinarum AIB39 and E. gallinarum GS1 were studied to establish whether the expression of vanC-1-mediated resistance may be inducible or constitutive. By growth curve analysis, strain AIB39 exhibited the same lag period (i.e., 1 to 1.5 h) whether it was subcultured to unsupplemented brain heart infusion broth or broth containing 6 mu g of vancomycin per mi, a growth pattern typical of constitutively expressed resistance, Use of high-performance liquid chromatography (HPLC) to separate peptidoglycan precursor extracts substantiated this finding because the serine-terminating pentapeptide precursor UDP-MurNAc-L-Ala-D-Glu-L-Lys-D-Ala-D-Ser was produced in the presence and absence of vancomycin, whereas no UDP-MurNAc-L-Ala-D-Glu-L-Lys-D-Ala-D-Ala was detected. In contrast, results with strain GSI were consistent with inducible expression, GS1 demonstrated a lag time that was 3 to 4 h longer when it was subcultured to vancomycin containing broth than when it was subcultured in unsupplemented broth. HPLC analysis showed that in the absence of vancomycin only UDP-MurNAc-L-Ala-D-Glu-L-Lys-D-Ala-D-Ala was detected, but in the presence of drug only UDP-MurNAc-L-Ala-D-Glu-L-Lys-D-Ala-D-Ser was found, Inducible expression of vanC-1-mediated resistance in E. gallinarum is consistent with recent findings suggesting the presence of at least two ligases in this species, Although vanC-1 may be intrinsic to E. gallinarum, our findings raise doubt regarding the natural mechanism of this gene's expression.
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页码:1480 / 1484
页数:5
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