INCORPORATION OF ARACHIDONIC-ACID IN A HUMAN CANCER GASTRIC TUMOR-CELL LINE (HGT) AT VARIOUS STAGES OF CELL-PROLIFERATION

被引：14

作者：

DENIZOT, Y

NAJID, A

RIGAUD, M

机构：

[1] CNRS URA 1485, Faculté de Médecine, 87025 Limoges

来源：

CANCER LETTERS | 1993年 / 68卷 / 2-3期

关键词：

ARACHIDONIC ACID; GASTRIC CANCER CELL LINE; PHOSPHOLIPIDS; PROLIFERATION; TRIGLYCERIDES;

D O I：

10.1016/0304-3835(93)90147-2

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Human cancer gastric cells (HGT) in culture were labeled with [C-14]arachidonic acid. The kinetic of acylation of arachidonic acid was similar using cells after 3, 5 and 7 days of culture. However, the duration of the proliferation enhanced the acyltransfer to triglycerides and inhibited the transfer to phospholipids. After 8 h of incubation with [C-14]arachidonic acid the radioactivity was predominantly recovered in the phosphatidylcholine and phosphatidylethanolamine species. Prelabeling-chase experiments indicated a preferential incorporation of arachidonic acid into phosphatidylcholine followed by a transfer of arachidonate to phosphatidylethanolamine.

引用

页码：199 / 205

页数：7

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