EXPRESSION OF ERYTHROID-DIFFERENTIATION FACTOR (EDF) IN CHINESE-HAMSTER OVARY CELLS

被引：55

作者：

MURATA, M ^{[1
]}

ONOMICHI, K ^{[1
]}

ETO, Y ^{[1
]}

SHIBAI, H ^{[1
]}

MURAMATSU, M ^{[1
]}

机构：

[1] UNIV TOKYO, FAC MED, DEPT BIOCHEM, BUNKYO KU, TOKYO 113, JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1988年 / 151卷 / 01期

关键词：

D O I：

10.1016/0006-291X(88)90583-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Plasmid DNA containing EDF subunit cDNA and mouse dihydrofolate reductase (DHFR) cDNA was transfected into CHO DHFR- cells by the calcium-phosphate method. DHFR positive transformants secreted recombinant EDF (r-EDF) constitutively in an active form and accumulated it in the conditioned medium. Furthermore, cells which were resistant to methotrexate (MTX: 0.5 .mu.M) secreted r-EDF up to 1 .mu.g/ml. r-EDF was identical to natural EDF (n-EDF) produced by human acute monocytic leukemia cell line, THP-1, as regards its dimeric structure and a biological activity.

引用

页码：230 / 235

页数：6

共 9 条

[1]

CHIRGWIN JM, 1979, BIOCHEMISTRY-US, V18, P5298

[2]

ETO Y, 1987, BIOCHEM BIOPH RES CO, V142, P1098

[3] NEW TECHNIQUE FOR ASSAY OF INFECTIVITY OF HUMAN ADENOVIRUS 5 DNA [J].

GRAHAM, FL ;

VANDEREB, AJ .

VIROLOGY, 1973, 52 (02) :456-467

[4] RNA MOLECULAR-WEIGHT DETERMINATIONS BY GEL-ELECTROPHORESIS UNDER DENATURING CONDITIONS, A CRITICAL RE-EXAMINATION [J].

LEHRACH, H ;

DIAMOND, D ;

WOZNEY, JM ;

BOEDTKER, H .

BIOCHEMISTRY, 1977, 16 (21) :4743-4751

[5] STRUCTURE OF 2 HUMAN OVARIAN INHIBINS [J].

MASON, AJ ;

NIALL, HD ;

SEEBURG, PH .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1986, 135 (03) :957-964

[6]

MURATA M, IN PRESS P NATL ACAD

[7] EXPRESSION OF AMPLIFIED CDNA AND GENOMIC SEQUENCES ENCODING HUMAN INTERLEUKIN-2 IN CHINESE-HAMSTER OVARY CELLS [J].

ONOMICHI, K ;

ETO, Y ;

SHIBAI, H .

JOURNAL OF BIOCHEMISTRY, 1987, 102 (01) :123-131

[8] ESTABLISHMENT AND CHARACTERIZATION OF A HUMAN ACUTE MONOCYTIC LEUKEMIA-CELL LINE (THP-1) [J].

TSUCHIYA, S ;

YAMABE, M ;

YAMAGUCHI, Y ;

KOBAYASHI, Y ;

KONNO, T ;

TADA, K .

INTERNATIONAL JOURNAL OF CANCER, 1980, 26 (02) :171-176

[9] PURIFICATION AND CHARACTERIZATION OF AN FSH RELEASING PROTEIN FROM PORCINE OVARIAN FOLLICULAR-FLUID [J].

VALE, W ;

RIVIER, J ;

VAUGHAN, J ;

MCCLINTOCK, R ;

CORRIGAN, A ;

WOO, W ;

KARR, D ;

SPIESS, J .

NATURE, 1986, 321 (6072) :776-779

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