SELECTION, RECOMBINATION, AND G-]A HYPERMUTATION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 GENOMES

被引:308
作者
VARTANIAN, JP
MEYERHANS, A
ASJO, B
WAINHOBSON, S
机构
[1] INST PASTEUR,RETROVIROL MOLEC LAB,F-75724 PARIS,FRANCE
[2] KAROLINSKA INST,DEPT VIROL,S-10401 STOCKHOLM 60,SWEDEN
关键词
D O I
10.1128/JVI.65.4.1779-1788.1991
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Human immunodeficiency virus type 1 (HIV-1) isolates are genetically so heterogeneous that they must be described in terms of populations of related but distinct genomes called quasispecies. A recent study of the influence of ex vivo culturing on HIV-1 quasispecies demonstrated that usually low-abundance genomes outgrew the more prominent forms. Here it is shown that multiple passages of an HIV-1 isolate on peripheral blood mononuclear cells resulted in the outgrowth of very minor forms. A single passage of equal proportions of supernatants to either of the established lymphocyte and monocyte cell lines Molt-3 and U937-2, respectively, resulted in the isolation of different sets of minor forms. Recombination between component sequences was observed. Extensive and monotonous base substitutions of G --> A (G --> A hypermutation) were evident in many sequences. A strong preference for the transition within the GpA dinucleotide was observed. Dislocation mutagenesis, in this case, a - 1 slippage or dislocation of the primer with respect to the template, during DNA synthesis by the HIV-1 reverse transcriptase would explain this bias. When the consequences of polymerase errors, recombination, hypermutation, and instability are added to the genetic description of HIV-1, the real complexity of this virus starts to become apparent.
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收藏
页码:1779 / 1788
页数:10
相关论文
共 46 条
  • [1] GENETIC-VARIABILITY OF THE AIDS VIRUS - NUCLEOTIDE-SEQUENCE ANALYSIS OF 2 ISOLATES FROM AFRICAN PATIENTS
    ALIZON, M
    WAINHOBSON, S
    MONTAGNIER, L
    SONIGO, P
    [J]. CELL, 1986, 46 (01) : 63 - 74
  • [2] ASJO B, 1986, LANCET, V2, P660
  • [3] BIASED HYPERMUTATION OF VIRAL-RNA GENOMES COULD BE DUE TO UNWINDING MODIFICATION OF DOUBLE-STRANDED-RNA
    BASS, BL
    WEINTRAUB, H
    CATTANEO, R
    BILLETER, MA
    [J]. CELL, 1989, 56 (03) : 331 - 331
  • [4] BEBENEK K, 1989, J BIOL CHEM, V264
  • [5] BUFFER GRADIENT GELS AND S-35 LABEL AS AN AID TO RAPID DNA-SEQUENCE DETERMINATION
    BIGGIN, MD
    GIBSON, TJ
    HONG, GF
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (13): : 3963 - 3965
  • [6] DNA METHYLATION AND THE FREQUENCY OF CPG IN ANIMAL DNA
    BIRD, AP
    [J]. NUCLEIC ACIDS RESEARCH, 1980, 8 (07) : 1499 - 1504
  • [7] RELIABLE ISOLATION OF HUMAN IMMUNODEFICIENCY VIRUS FROM CULTURES OF NATURALLY INFECTED CD4+ T-CELLS
    BRINCHMANN, JE
    GAUDERNACK, G
    THORSBY, E
    JONASSEN, TO
    VARTDAL, F
    [J]. JOURNAL OF VIROLOGICAL METHODS, 1989, 25 (03) : 293 - 300
  • [8] BRINCHMANN JE, 1990, J IMMUNOL, V144, P2961
  • [9] A SPECIFIC MISMATCH REPAIR EVENT PROTECTS MAMMALIAN-CELLS FROM LOSS OF 5-METHYLCYTOSINE
    BROWN, TC
    JIRICNY, J
    [J]. CELL, 1987, 50 (06) : 945 - 950
  • [10] REPEAT-INDUCED G-C TO A-T MUTATIONS IN NEUROSPORA
    CAMBARERI, EB
    JENSEN, BC
    SCHABTACH, E
    SELKER, EU
    [J]. SCIENCE, 1989, 244 (4912) : 1571 - 1575