HIGH-SEQUENCE CONSERVATION AMONG THE UNITED-STATES BLUETONGUE VIRUSES COGNATE M2-GENES WHICH ENCODE THE NONSTRUCTURAL NS1-TUBULE PROTEIN

被引:19
作者
HWANG, GY
CHIOU, JF
YANG, YY
LI, JKK
机构
[1] UTAH STATE UNIV, GRAD PROGRAM MOLEC BIOL, LOGAN, UT 84322 USA
[2] NATL TAIWAN UNIV, COLL MED, GRAD INST MICROBIOL, TAIPEI, TAIWAN
[3] UTAH STATE UNIV, DEPT BIOL UMC 5500, LOGAN, UT 84322 USA
关键词
D O I
10.1006/viro.1993.1036
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Full-length cDNA copies of the M2 gene of BTV-2, -11, and -13 serotypes obtained by a modified polymerase chain reaction (Clamp-R) were cloned into pUC19 plasmid. The entire nucleotide sequences of each M2 gene were determined and compared with BTV-10 and BTV-17, thus completing the sequencing of these cognate M2 gene segments from all five U.S. BTV serotypes. Each M2 segment contained 1769 nucleotides, a single open reading frame (ORF) with an initiation codon at nucleotides 35-37, and a termination codon at nucleotides 1691-1693. This ORF can encode the 552-amino-acid NS1 protein (64 KDa) which has an isoelectric point of 7. Analyses of the nucleotide and deduced amino acid sequences of the five U.S. BTV serotypes indicated that the most recently isolated BTV-2 serotype was more distantly related than BTV-10, -11, -13, or -17. Analyses of the evolutionary relatedness of the cognate M2 genes by codon positions indicate that the rate of mismatch accumulations in the first and second base codon positions are less than 4%. However, the mismatch accumulations in the third base codon position are quite evident (23%) when BTV-2 serotype was compared with the other U.S. BTV serotypes. This suggests that BTV-2 has separated from the other four U.S. serotypes long before they themselves diverged. These data also indicate that the five U.S. BTV serotypes were apparently derived from two distinct gene pools that reflected geographic distribution in North America. © 1993 Academic Press, Inc.
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页码:321 / 327
页数:7
相关论文
共 33 条
[1]  
[Anonymous], 1990, Curr Top Microbiol Immunol, V162, P1
[2]  
BARBER TL, 1979, AM J VET RES, V40, P1654
[3]   2 ELECTROPHEROTYPES OF BLUETONGUE VIRUS SEROTYPE-2 FROM NATURALLY INFECTED CALVES [J].
COLLISSON, EW ;
BARBER, TL ;
GIBBS, EPJ ;
GREINER, EC .
JOURNAL OF GENERAL VIROLOGY, 1985, 66 (JUN) :1279-1286
[4]   LOCALIZATION OF THE NONSTRUCTURAL PROTEIN NS1 IN BLUETONGUE VIRUS-INFECTED CELLS AND ITS PRESENCE IN VIRUS-PARTICLES [J].
EATON, BT ;
HYATT, AD ;
WHITE, JR .
VIROLOGY, 1988, 163 (02) :527-537
[5]   PROGRESSIVE SEQUENCE ALIGNMENT AS A PREREQUISITE TO CORRECT PHYLOGENETIC TREES [J].
FENG, DF ;
DOOLITTLE, RF .
JOURNAL OF MOLECULAR EVOLUTION, 1987, 25 (04) :351-360
[6]  
GIBBS EPJ, 1983, AM J VET RES, V44, P2226
[7]   IDENTIFICATION OF BLUETONGUE VIRUS TYPE-17 GENOME SEGMENTS CODING FOR POLYPEPTIDES ASSOCIATED WITH VIRUS NEUTRALIZATION AND INTERGROUP REACTIVITY [J].
GRUBMAN, MJ ;
APPLETON, JA ;
LETCHWORTH, GJ .
VIROLOGY, 1983, 131 (02) :355-366
[8]   CLUSTAL - A PACKAGE FOR PERFORMING MULTIPLE SEQUENCE ALIGNMENT ON A MICROCOMPUTER [J].
HIGGINS, DG ;
SHARP, PM .
GENE, 1988, 73 (01) :237-244
[9]  
HOFF GL, 1973, J AM VET MED ASSOC, V163, P565
[10]   PROTEIN-SYNTHESIS IN BLUETONGUE VIRUS-INFECTED CELLS [J].
HUISMANS, H .
VIROLOGY, 1979, 92 (02) :385-396