ALLOSTERIC PROPERTIES OF HEMOGLOBIN BETA-41 (C7) PHE-]TYR - A STABLE, LOW-OXYGEN-AFFINITY VARIANT SYNTHESIZED IN ESCHERICHIA-COLI

被引：23

作者：

BAUDIN, V ^{[1
]}

PAGNIER, J ^{[1
]}

LACAZE, N ^{[1
]}

BIHOREAU, MT ^{[1
]}

KISTER, J ^{[1
]}

MARDEN, M ^{[1
]}

KIGER, L ^{[1
]}

POYART, C ^{[1
]}

机构：

[1] HOP BICETRE,INSERM,U299,F-94275 LE KREMLIN BICETR,FRANCE

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1992年 / 1159卷 / 02期

关键词：

HB FUNCTION; ALLOSTERIC TRANSITION; PROTEIN ENGINEERING; OXYGEN CARRIER;

D O I：

10.1016/0167-4838(92)90029-D

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In human deoxy haemoglobin, the alpha42(C7)Tyr-residue is hydrogen-bonded to beta99(G1)Asp which stabilizes the low-oxygen-affinity deoxy conformation. We engineered a haemoglobin with Tyr for Phe at the homologous C7 position in beta-chains. The oxygen affinity of the variant is decreased about two-fold relative to Hb A while keeping similar K(R) and K(T) values. This mutant may be a candidate for the development of an artificial oxygen carrier, as it would not require an external effector for significant oxygen unloading in vivo.

引用

页码：223 / 226

页数：4

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