THE NEUROPROTECTIVE KAPPA-OPIOID CI-977 ALTERS GLUTAMATE-INDUCED CALCIUM SIGNALING IN-VITRO

被引：10

作者：

DECOSTER, MA

CONOVER, JR

HUNTER, JC

TORTELLA, FC

机构：

[1] WALTER REED ARMY MED CTR,WALTER REED ARMY INST RES,DEPT MED NEUROSCI,WASHINGTON,DC 20307

[2] PARKE DAVIS NEUROSCI RES CTR,CAMBRIDGE CB2 2QB,ENGLAND

来源：

NEUROREPORT | 1994年 / 5卷 / 17期

关键词：

GLUTAMATE NEUROTOXICITY; INTRACELLULAR CALCIUM; FLUO-3; NEURONS; CELLULAR IMAGING; KAPPA-OPIOID; NEUROPROTECTION; CONFOCAL MICROSCOPY;

D O I：

10.1097/00001756-199411000-00025

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

THE effect of the neuroprotective kappa opioid agonist CI-977 on glutamate (GLU)-stimulated calcium signaling was studied in individual primary rat cortical neurons. Using laser scanning confocal microscopy and the fluorescent calcium probe fluo-3, both the sustained and biphasic intracellular calcium concentration [Ca2+](1) changes induced by GLU (20-40 mu M) were altered by CI-977 (25-100 nM), thereby shifting the neuronal population response from unbuffered to buffered patterns of [Ca2+](i) flux. This effect was consistent with the previously demonstrated neuroprotective action of CI-977 against glutamate toxicity in vitro. The effect of CI-977 in altering GLU-induced [Ca2+](i) signaling was attenuated by naloxone, consistent with a neuroprotective action of CI-977 at opioid receptors, presumably of the kappa subtype.

引用

页码：2305 / 2310

页数：6

共 18 条

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