AN ELISA CAPTURE ASSAY FOR THE E7 TRANSFORMING PROTEINS OF HPV16 AND HPV18

被引：12

作者：

SELVEY, LA ^{[1
]}

DUNN, LA ^{[1
]}

MURRAY, B ^{[1
]}

TINDLE, RW ^{[1
]}

FRAZER, IH ^{[1
]}

机构：

[1] UNIV QUEENSLAND,PRINCESS ALEXANDRA HOSP,PAPILLOMAVIRUS RES UNIT,LIONS HUMAN IMMUNOL LABS,WOOLLOONGABBA,QLD 4102,AUSTRALIA

来源：

JOURNAL OF VIROLOGICAL METHODS | 1992年 / 37卷 / 02期

基金：

英国医学研究理事会;

关键词：

ELISA; PAPILLOMAVIRUSES; E7; PROTEIN; CERVIX CANCER;

D O I：

10.1016/0166-0934(92)90039-G

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

ELISA capture assays were established for the E7 transforming proteins of HPV16 and HPV18, based on a range of previously characterised polyclonal and monoclonal antibodies. No cross-reactivity was observed in the ELISAs between HPV18 E7 and HPV16 E7. Immunoreactive E7 protein (iE7) was measured in a series of HPV-transformed cell lines, and ranged from 0.6 to 17.7 ng iE7/mg cell protein. iE7 was labile at 22-degrees-C (t1/2 = 37 min) but relatively more stable at 4-degrees-C (t1/2 = 210 min). HPV 16 E7 protein at concentrations from 0.10 to 0.69 ng iE7/mg cell protein was detected in 5 of 13 smears from women with abnormal cervical cytology. Assay of E7 protein may play a role in the detection of HPV-induced cervical lesions with malignant potential.

引用

页码：119 / 128

页数：10

共 14 条

[1] CONTINUED EXPRESSION OF HPV-16 E7 PROTEIN IS REQUIRED FOR MAINTENANCE OF THE TRANSFORMED PHENOTYPE OF CELLS CO-TRANSFORMED BY HPV-16 PLUS EJ-RAS [J].

CROOK, T ;

MORGENSTERN, JP ;

CRAWFORD, L ;

BANKS, L .

EMBO JOURNAL, 1989, 8 (02) :513-519

[2]

DOEBERITZ MV, 1990, CANCER RES, V50, P3730

[3]

GISSMANN L, 1986, 21 COLD SPRING HARB, P217

[4] HPV16 E6-PROTEINS AND E7-PROTEINS COOPERATE TO IMMORTALIZE HUMAN FORESKIN KERATINOCYTES [J].

HAWLEYNELSON, P ;

VOUSDEN, KH ;

HUBBERT, NL ;

LOWY, DR ;

SCHILLER, JT .

EMBO JOURNAL, 1989, 8 (12) :3905-3910

[5] 2 IMMUNODOMINANT REGIONS OF THE HUMAN PAPILLOMAVIRUS TYPE-16 E7-PROTEIN ARE MASKED IN THE NUCLEI OF MONKEY COS-1 CELLS [J].

KANDA, T ;

ZANMA, S ;

WATANABE, S ;

FURUNO, A ;

YOSHIIKE, K .

VIROLOGY, 1991, 182 (02) :723-731

[6] AGE AND TIME TRENDS IN THE PREVALENCE OF CERVICAL INTRAEPITHELIAL NEOPLASIA ON PAPANICOLAOU SMEAR TESTS, 1970-1988 [J].

MITCHELL, H ;

MEDLEY, G .

MEDICAL JOURNAL OF AUSTRALIA, 1990, 152 (05) :252-255

[7] COMPLEX-FORMATION OF HUMAN PAPILLOMAVIRUS-E7 PROTEINS WITH THE RETINOBLASTOMA TUMOR SUPPRESSOR GENE-PRODUCT [J].

MUNGER, K ;

WERNESS, BA ;

DYSON, N ;

PHELPS, WC ;

HARLOW, E ;

HOWLEY, PM .

EMBO JOURNAL, 1989, 8 (13) :4099-4105

[8]

MUNGER K, 1989, J VIROL, V63, P4417

[9] CHARACTERIZATION OF HUMAN KERATINOCYTE X HELA SOMATIC-CELL HYBRIDS [J].

PEEHL, DM ;

STANBRIDGE, EJ .

INTERNATIONAL JOURNAL OF CANCER, 1981, 27 (05) :625-635

[10] IDENTIFICATION OF EARLY PROTEINS OF THE HUMAN PAPILLOMA VIRUSES TYPE-16 (HPV 16) AND TYPE-18 (HPV 18) IN CERVICAL-CARCINOMA CELLS [J].

SEEDORF, K ;

OLTERSDORF, T ;

KRAMMER, G ;

ROWEKAMP, W .

EMBO JOURNAL, 1987, 6 (01) :139-144

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