ALL-OR-NONE MECHANISM OF THE MOLTEN GLOBULE UNFOLDING

被引：102

作者：

UVERSKY, VN ^{[1
]}

SEMISOTNOV, GV ^{[1
]}

PAIN, RH ^{[1
]}

PTITSYN, OB ^{[1
]}

机构：

[1] UNIV NEWCASTLE UPON TYNE,DEPT BIOCHEM & GENET,NEWCASTLE TYNE NE2 4HH,ENGLAND

来源：

FEBS LETTERS | 1992年 / 314卷 / 01期

关键词：

PROTEIN FOLDING; MOLTEN GLOBULE; SIZE EXCLUSION CHROMATOGRAPHY; CARBONIC ANHYDRASE-B; BETA-LACTAMASE;

D O I：

10.1016/0014-5793(92)81468-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Gdm-HCl-induced unfolding of bovine carbonic anhydrase B and S. aureus beta-lactamase was studied at 4-degrees-C by a variety of methods. With the use of FPLC it has been shown that within the transition from the molten globule to the unfolded state the distribution function of molecular dimensions is bimodal. This means that equilibrium intermediates between the molten globule and the unfolded states are absent, i.e. the molten globule unfolding follows the 'all-or-none' mechanism.

引用

页码：89 / 92

页数：4

共 21 条

[1]

ARMSTRONG JM, 1966, J BIOL CHEM, V241, P5137

[2] THE MOLTEN GLOBULE STATE IS INVOLVED IN THE TRANSLOCATION OF PROTEINS ACROSS MEMBRANES [J].

BYCHKOVA, VE ;

PAIN, RH ;

PTITSYN, OB .

FEBS LETTERS, 1988, 238 (02) :231-234

[3]

CHRISTENSEN H, 1991, EUR BIOPHYS J, V19, P221, DOI 10.1007/BF00183530

[4] USE OF HIGH-SPEED SIZE-EXCLUSION CHROMATOGRAPHY FOR THE STUDY OF PROTEIN FOLDING AND STABILITY [J].

CORBETT, RJT ;

ROCHE, RS .

BIOCHEMISTRY, 1984, 23 (08) :1888-1894

[5] MOLTEN-GLOBULE STATE ACCUMULATES IN CARBONIC-ANHYDRASE FOLDING [J].

DOLGIKH, DA ;

KOLOMIETS, AP ;

BOLOTINA, IA ;

PTITSYN, OB .

FEBS LETTERS, 1984, 165 (01) :88-92

[6] ALPHA-LACTALBUMIN - COMPACT STATE WITH FLUCTUATING TERTIARY STRUCTURE [J].

DOLGIKH, DA ;

GILMANSHIN, RI ;

BRAZHNIKOV, EV ;

BYCHKOVA, VE ;

SEMISOTNOV, GV ;

VENYAMINOV, SY ;

PTITSYN, OB .

FEBS LETTERS, 1981, 136 (02) :311-315

[7]

DOLGIKH DA, 1985, EUR BIOPHYS J BIOPHY, V13, P109, DOI 10.1007/BF00256531

[8] DIRECT SPECTROPHOTOMETRIC ASSAY FOR PENICILLIN BETA-LACTAMASE (PENICILLINASE) [J].

JANSSON, JAT .

BIOCHIMICA ET BIOPHYSICA ACTA, 1965, 99 (01) :171-&

[9] THE MOLTEN GLOBULE STATE AS A CLUE FOR UNDERSTANDING THE FOLDING AND COOPERATIVITY OF GLOBULAR-PROTEIN STRUCTURE [J].

KUWAJIMA, K .

PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS, 1989, 6 (02) :87-103

[10] CHAPERONIN-MEDIATED PROTEIN FOLDING AT THE SURFACE OF GROEL THROUGH A MOLTEN GLOBULE-LIKE INTERMEDIATE [J].

MARTIN, J ;

LANGER, T ;

BOTEVA, R ;

SCHRAMEL, A ;

HORWICH, AL ;

HARTL, FU .

NATURE, 1991, 352 (6330) :36-42

← 1 2 3 →