PHOTOLYTIC OXIDATION OF REDUCED NICOTINAMIDE ADENINE-DINUCLEOTIDE

被引：14

作者：

SHARMA, A

机构：

[1] Division of Molecular Biophysics, Optical Diagnostics Biotechnology Centre, Cranfield Institute of Technology Cranfield

来源：

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | 1992年 / 48卷 / 06期

关键词：

D O I：

10.1016/0584-8539(92)80086-C

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

A photo-reaction between thionine dye and NADH has been observed which leads to the oxidation of the co-factor. Thionine (fluorescent), which is converted into semi/leuko thionine (non-fluorescent) during light reaction, fully recovers during the dark reaction. Analytical applications of this discovery are discussed. This reaction opens the door to the measurement of NADH using the fluorescence quenching of thionine and to the construction of several optical biosensors.

引用

页码：893 / 897

页数：5

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