PROTEIN REFOLDING USING AQUEOUS 2-PHASE SYSTEMS

被引：22

作者：

FORCINITI, D

机构：

[1] Chemical Engineering Department, University of Missouri-Rolla, Rolla

来源：

JOURNAL OF CHROMATOGRAPHY A | 1994年 / 668卷 / 01期

关键词：

D O I：

10.1016/0021-9673(94)80096-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We present a novel refolding technique in which aqueous two-phase systems are used to both dissociate protein aggregates (inclusion bodies) and to refold the protein in only one operation. Three denaturant salts (sodium thiocyanate, calcium chloride, and lithium bromide) were used as phase-forming salts with poly(ethylene glycol) (PEG). We have tested the technique by dissolving carbonic anhydrase II aggregates and refolding the denatured protein in PEG-NaCl-NaSCN systems.

引用

页码：95 / 100

页数：6

共 20 条

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