MODIFIED POLY(GLYCIDYL METHACRYLATE-CO-ETHYLENE DIMETHACRYLATE) CONTINUOUS ROD COLUMNS FOR PREPARATIVE-SCALE ION-EXCHANGE CHROMATOGRAPHY OF PROTEINS

被引：180

作者：

SVEC, F

FRECHET, JMJ

机构：

[1] Cornell University, Department of Chemistry, Baker Laboratory, Ithaca

来源：

JOURNAL OF CHROMATOGRAPHY A | 1995年 / 702卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(94)01021-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A continuous rod of porous poly(glycidyl methacrylate-co-ethylene dimethacrylate) has been prepared by a free radical polymerization within the confines of a 300 x and mm I.D. chromatographic column. The epoxide groups of the rod have been modified by a reaction with diethylamine that affords ionizable functionalities required for the ion-exchange chromatographic mode. The properties of this rod column have been characterized and the column has been used successfully for the chromatographic separation of proteins. The column exhibits a dynamic capacity that exceeds 300 mg at a flow velocity of 200 cm/min. An excellent selectivity allows the separation of up to 300 mg of a protein mixture in a single run.

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页码：89 / 95

页数：7

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