A POINT MUTATION IN THE COAT PROTEIN ABOLISHES APHID TRANSMISSIBILITY OF A POTYVIRUS

被引:267
作者
ATREYA, CD [1 ]
RACCAH, B [1 ]
PIRONE, TP [1 ]
机构
[1] UNIV KENTUCKY, DEPT PLANT PATHOL, LEXINGTON, KY 40546 USA
关键词
D O I
10.1016/0042-6822(90)90389-9
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A nonaphid transmissible (NAT) variant of tobacco vein mottling virus (TVMV) was used to test the hypothesis that the viral coat protein (CP) plays a role in determining aphid transmissibility. Comparison of the nucleotide sequences in the coat protein cistron of an aphid transmissible isolate (TVMV-AT) with that of TVMV-NAT revealed a single nucleotide difference (G → A) at position 8445; this alters a single amino acid residue (G → E) at position 2747. A cDNA fragment representing the CP region of TVMV-NAT was substituted into the CP region of a full-length cDNA clone of TVMV-AT, and transcribed RNA was inoculated to tobacco plants. Aphids were unable to transmit the resultant hybrid virus which had the TVMV-NAT coat protein, although the concentration and infectivity of the hybrid virus in the source plants were similar to those of TVMV-AT. This is the first direct demonstration that a CP mutation affects aphid transmissibility of a potyvirus. © 1990.
引用
收藏
页码:161 / 165
页数:5
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