DNA MODIFIED BY PLATINUM DERIVATIVES CANNOT ADOPT THE A-FORM

被引：11

作者：

BALCAROVA, Z ^{[1
]}

BRABEC, V ^{[1
]}

机构：

[1] CZECHOSLOVAK ACAD SCI, INST BIOPHYS, KRALOVOPOLSKA 135, CS-61265 BRNO, CZECHOSLOVAKIA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1986年 / 867卷 / 1-2期

关键词：

D O I：

10.1016/0167-4781(86)90026-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

DNA fragments from chicken erythrocytes were modified by cis-diamminedichloroplatinum(II), its trans-isomer and chlorodiethylenetriaminoplatinum(II) chloride. The conformation of the modified DNA fragments in ethanolic solutions was studied by circular dichroism spectroscopy. Non-modified DNA adopted the A-form in 81% ethanol. The modification of DNA by the three platinum compounds inhibited the B to A transition of DNA induced by high concentrations of ethanol roughly to the same extent. The results support the view that the binding of the platinum complexes to B-DNA lowers the conformational freedom of DNA so that it cannot acquire the A-conformation.

引用

页码：31 / 35

页数：5

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