TRANSFORMATION OF THE OOMYCETE PATHOGEN PHYTOPHTHORA-MEGASPERMA F-SP GLYCINEA OCCURS BY DNA INTEGRATION INTO SINGLE OR MULTIPLE CHROMOSOMES

被引:48
作者
JUDELSON, HS
COFFEY, MD
ARREDONDO, FR
TYLER, BM
机构
[1] UNIV CALIF DAVIS,DEPT PLANT PATHOL,DAVIS,CA 95616
[2] UNIV CALIF DAVIS,DEPT VEGETABLE CROPS,DAVIS,CA 95616
[3] UNIV CALIF RIVERSIDE,DEPT PLANT PATHOL,RIVERSIDE,CA 92521
关键词
TRANSFORMATION; OOMYCETE; PHYTOPHTHORA-MEGASPERMA; CHROMOSOME SEPARATION-EX;
D O I
10.1007/BF00351498
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
A procedure for stable transformation was developed for Phytophthora megasperma f. sp. glycinea, an oomycete pathogen of soybean. Transformants were obtained using a bacterial hygromycin resistance gene fused to a promoter and terminator from the ham34 gene of another oomycete, Bremia lactucae. Vector DNA, alone or complexed to cationic liposomes, was introduced into protoplasts using polyethylene glycol and CaCl2. DNA and RNA hybridization, and phosphotransferase assays, confirmed the presence and expression of vector DNA in the transformants. Hybridization to electrophoretically separated chromosomes of P. m. glycinea showed that vector DNA had integrated into only one chromosome in four transformants, and into multiple chromosomes in one transformant.
引用
收藏
页码:211 / 218
页数:8
相关论文
共 30 条
[1]   GENETIC-TRANSFORMATION OF THE PLANT-PATHOGENS PHYTOPHTHORA-CAPSICI AND PHYTOPHTHORA-PARASITICA [J].
BAILEY, AM ;
MENA, GL ;
HERRERAESTRELLA, L .
NUCLEIC ACIDS RESEARCH, 1991, 19 (15) :4273-4278
[2]  
Buzzell R. I., 1987, Soybean Genetics Newsletter, V14, P79
[3]   HETEROLOGOUS GENE-EXPRESSION IN THE BASIDIOMYCETE FUNGUS COPRINUS-CINEREUS [J].
CASSELTON, LA ;
HERCE, AD .
CURRENT GENETICS, 1989, 16 (01) :35-40
[4]   SEPARATION OF LARGE DNA-MOLECULES BY CONTOUR-CLAMPED HOMOGENEOUS ELECTRIC-FIELDS [J].
CHU, G ;
VOLLRATH, D ;
DAVIS, RW .
SCIENCE, 1986, 234 (4783) :1582-1585
[5]   TRANSFORMATION IN FUNGI [J].
FINCHAM, JRS .
MICROBIOLOGICAL REVIEWS, 1989, 53 (01) :148-170
[6]   THE COMPLEMENTARY GENIC SYSTEMS IN FLAX AND FLAX RUST [J].
FLOR, HH .
ADVANCES IN GENETICS INCORPORATING MOLECULAR GENETIC MEDICINE, 1956, 8 :29-54
[7]   NONRECIPROCAL EXCHANGES OF INFORMATION BETWEEN DNA DUPLEXES COINJECTED INTO MAMMALIAN-CELL NUCLEI [J].
FOLGER, KR ;
THOMAS, K ;
CAPECCHI, MR .
MOLECULAR AND CELLULAR BIOLOGY, 1985, 5 (01) :59-69
[8]   SEQUENCE-ANALYSIS OF THE SMALL SUBUNIT RIBOSOMAL-RNAS OF 3 ZOOSPORIC FUNGI AND IMPLICATIONS FOR FUNGAL EVOLUTION [J].
FORSTER, H ;
COFFEY, MD ;
ELWOOD, H ;
SOGIN, ML .
MYCOLOGIA, 1990, 82 (03) :306-312
[9]   TRANSIENT EXPRESSION OF GENES IN THE OOMYCETE PHYTOPHTHORA-INFESTANS USING BREMIA-LACTUCAE REGULATORY SEQUENCES [J].
JUDELSON, HS ;
MICHELMORE, RW .
CURRENT GENETICS, 1991, 19 (06) :453-459
[10]   REGULATORY SEQUENCES FOR EXPRESSING GENES IN OOMYCETE FUNGI [J].
JUDELSON, HS ;
TYLER, BM ;
MICHELMORE, RW .
MOLECULAR & GENERAL GENETICS, 1992, 234 (01) :138-146